电针“三阴交穴”调节自然围绝经期大鼠雌激素及单胺类神经递质的实验研究

目的研究电针"三阴交穴"对自然围绝经期大鼠单胺类神经递质的影响。方法将大鼠分为正常组、模型组与电针组,电针组大鼠在三阴交处进行电针,隔日1次,共治疗15次。其他两组大鼠进行常规饲养,不进行针刺,隔日抓取。酶免方法(Elisa)检测大鼠卵巢雌二醇(E2)水平和海马部位的单胺类神经递质含量。结果 Elisa结果提示,与正常组比较,模型组大鼠卵巢E2含量降低了59.17%(P0.01)。与模型组比较,治疗组大鼠卵巢E2含量显著增高了65.49%(P0.01),但仍未恢复至正常水平(P0.01)。中枢神经递质的结果显示:与正常组比较,模型组大鼠海马区五羟色胺(5-HT)含量升高了34.35%,NE、DA分别下降了33.86%和34.38%(P0.01)。与模型组比较,治疗组大鼠5-HT含量明显降低了11.13%(P0.05),NE显著上调了19.18%(P0.05),差异有统计学意义。治疗组DA含量虽升高了7.59%,但差异无统计学意义(P0.05)。与正常组比较,模型组的5-HT/NE的比值显著升高(P0.01)。与模型组比较,治疗组的5-HT/NE的比值显著降低了23.47%(P0.01)。结论电针"三阴交"穴可以调节自然围绝经期大鼠的性激素水平及中枢单胺类神经递质的水平。

Experimental study on regulation of estrogen and monoamine neurotransmitters in natural climacteric rat-through electroacupuncture at"Sanyinjiao"

Objective To investigate the effects of electroacupuncture at ＂ Sanyinjiao＂ on estrogen and monoamine neurotransmitters of natural menopausal rats. Methods Rats were divided into normal group,model group and electroacupuncture group randomly. Electro acupuncture group rats were acupunctured in Sanyinjiao for 15 therapies,each therapy was given once every other day. The rats inother two groups were fed as the model groupbut without acupuncture. ELISA method was applied to detect the levels of ovarian estradiol（ E2） and monoamine neurotransmitters in hippocampus. Results Compared with the normal group,the concentrationof ovarianE2 in model grouprats decreased by 59. 17%（ P 〈0. 01）. After the treatment of theelectro- acupuncture,the level of E2 was significantly increased by 65. 49%（ P 〈0. 01） compared with the model group,but the level was not restored to the normal level（ P 〈0. 01）. The central neurotransmitter results showthat compared with the normal group,the content of5-HT in hippocampus in the model group rats increased by 34. 35%. The levels of NE,DA were decreased by 33.86% and 34. 38% respectively（ P 〈0. 01） in model group rats. The content of 5-HT in the treatment group rats were decreased by 11. 13%（ P 〈0. 05） compared with the model grouprats. However,NE was significantly up-regulated by19. 18%（ P 〈0. 05） at the same time. DA intreatment group rats was increased by 7. 59%,but had no statistical significance with the model group（ P 〉0. 05）. Compared with the normal group,the ratio of 5-HT /NE in model group ratsincreased significantly（ P 〈0. 01）. After the treatment of the electro-acupuncture,the ratio 5-HT /NE of the treatment group decreased significantly by23. 47%（ P 〈0. 01）. Conclusion Electroacupuncture at ＂ Sanyinjiao＂ point can regulate sex hormone levels and monoamine neurotransmitter levels in the natural climacteric rats.