| C-kit原癌基因在白血病细胞上的表达分析 |
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| 引用本文： | 陈葆国,李伯利,干灵红,罗文达.C-kit原癌基因在白血病细胞上的表达分析[J].中国实验诊断学,2004,8(2):125-127. |
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| 作者姓名： | 陈葆国 李伯利 干灵红 罗文达 |
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| 作者单位： | 浙江温州医学院附属台州医院,中心实验室,浙江,临海市,317000 |
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| 摘 要： | 目的 研究C kit(CD117)原癌基因在白血病细胞上的表达规律和意义。方法 采用CD4 5 SSC双参数 (对数 )散点图设门法进行三色流式细胞术分析。结果 急性髓细胞白血病 (AML)患者CD117表达率为 5 9 6 % ;慢性髓细胞白血病 (CML)CD117表达率为 16 7% ,且都为急变期 ;急性淋巴细胞性白血病 (ALL)极少表达 ,慢性淋巴细胞性白血病 (CLL)不表达 ;干细胞性白血病 (AUL)、双表型白血病 (BAL)高表达。结论 CD117有助于鉴别诊断髓系白血病 ,并能作为慢粒病程监测的指标
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| 关 键 词： | 白血病 C-kit原癌基因 流式细胞术 |
| 文章编号： | 1007-4287(2004)02-0125-03 |
| 修稿时间： | 2003年8月25日 |
Expression of C-kit proto-oncogene in leukemia cells |
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| CHEN Bao-Guo,LI Bo-Li,GAN Li-Hong,et al..Expression of C-kit proto-oncogene in leukemia cells[J].Chinese Journal of Laboratory Diagnosis,2004,8(2):125-127. |
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| Authors: | CHEN Bao-Guo LI Bo-Li GAN Li-Hong |
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| Abstract: | Objective To study the expressive regularity of C-kit(CD117)proto-onco Abstractgene in keukemic cells and it significances.Methods CD45/SSCdouble parameter(log scatter diagram gating was used by means of third-color flow cytometry analysis.Results CD117 was positive in 59 6% of patients in acute myelocytic leukemia, 16 7% in chromic myelocytic leukemia paitents in myeloid blastic crisis; the expression of CD117 were low in acute lymphocytic leukemia; All of the patients with chromic lymphocytic leukemia were CD117 the negative; the expressions of CD117 were high in undifferentiaded acute leukemia and mixed acute leukemia. Conclusion The expression of CD117 was used for distinguishing myeloid leukemia, and it is a useful index as progress monitoring in chromic myeloid leukemia. |
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| Keywords: | leukemia C-kit proto-oncogene flow cytometry |
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