| CpG002促进人胚肺成纤维细胞增殖及Ⅰ型胶原表达 |
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| 引用本文： | 李丽娜,王华,张永胜,王颖,吴秀丽,孙陆果,于永利,王丽颖.CpG002促进人胚肺成纤维细胞增殖及Ⅰ型胶原表达[J].中国实验诊断学,2010,14(3):352-354. |
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| 作者姓名： | 李丽娜 王华 张永胜 王颖 吴秀丽 孙陆果 于永利 王丽颖 |
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| 作者单位： | 1. 吉林大学白求恩医学院,分子生物学教研室,吉林,长春130021
2. 吉林大学白求恩医学院,免疫学教研室,吉林,长春130021 |
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| 摘 要： | 目的观察C型CoGODN（002）对人胚肺成纤维细胞增殖及表达Ⅰ型胶原的影响。方法采用人胚肺成纤维细胞（human embryonic lung fibroblast，HELF）作为靶细胞，以细胞计数的方法观察两种C型CoGODN对HELF细胞增殖的影响，以RT-PCR方法检测CpGODN诱导HELFⅠ型胶原mRNA的表达情况。结果两种C型CpGODN（CpG001和cpG002）均能明显刺激HELF增殖，但只有CpG002也同时表现出诱导HELF表达Ⅰ型胶原的作用。结论C型CpGODN002能促进人胚肺成纤维细胞增殖及分泌Ⅰ型胶原。
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| 关 键 词： | 人胚肺成纤维细胞 增殖 Ⅰ型胶原 CpG ODN |
CpG 002 promotes the proliferation and expression of type I collagen of human embryonic lung fibroblasts |
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| LI Li-na,WANG Hua,ZHANG Yong-sheng,et al..CpG 002 promotes the proliferation and expression of type I collagen of human embryonic lung fibroblasts[J].Chinese Journal of Laboratory Diagnosis,2010,14(3):352-354. |
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| Authors: | LI Li-na WANG Hua ZHANG Yong-sheng |
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| Institution: | LI Li-na,WANG Hua,ZHANG Yong-sheng,et al.(Department of Molecular Biology,Norman Bethune Medical College,Jilin University,Changchun 130021,China) |
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| Abstract: | Objective To study the effect of novel C-type CpG ODNs on proliferation and expression of type Ⅰ collagen by HELF cells. Methods HELF cells were used as target cells which were stimulated by CpG ODN（002） or CpG 001 in vitro. Then cell proliferation was analyzed by cell counting and mRNA level of type Ⅰ collagen was detected by RT-PCR. Results Both of the two CpG ODNs can promote the proliferation of HELF cells, but only CpG 002 can stimulate HELF cell to express type Ⅰ collagen simultaneously. Conclusion C type CpG ODN 002 can promote HPF cell to proliferate and express type Ⅰ collagen. |
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| Keywords: | CpG ODN |
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