| GFP在人脐血CD34~+细胞中的表达及意义 |
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| 引用本文： | 卢振霞,白元松,林玉梅.GFP在人脐血CD34~+细胞中的表达及意义[J].中国实验诊断学,2010,14(6):831-832. |
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| 作者姓名： | 卢振霞 白元松 林玉梅 |
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| 作者单位： | 吉林大学中日联谊医院,血液肿瘤科,吉林,长春,130033 |
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| 基金项目： | 中国博士后科学基金面上资助项目，留学回国人员科研启动基金项目资助课题 |
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| 摘 要： | 目的探讨慢病毒载体在CD34+脐血细胞(CBCs)中的基因转导效率,为基因治疗的临床应用提供关键材料。方法应用1型人类免疫缺陷病毒(HIV-1)改造而成的第三代自身失活(self-inactivating,SIN)慢病毒载体(lentiviralvector)系统,通过流式细胞术检测基因导入细胞百分比,评价该载体系统在人CBCs中的基因转导效率。结果 HIV载体的转导效率在95%以上。结论基于慢病毒载体基因转导的高效性,该载体系统可作为CD34+CBCs基因转导的极好工具。
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| 关 键 词： | 脐血 造血干/祖细胞 绿色荧光蛋白 慢病毒载体 基因转导效率 |
Green fluorescent protein gene transduction efficiency in human CD34+ cord blood cells |
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| LU Zhen-xia,BAI Yuan-song,LIN Yu-mei.Green fluorescent protein gene transduction efficiency in human CD34+ cord blood cells[J].Chinese Journal of Laboratory Diagnosis,2010,14(6):831-832. |
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| Authors: | LU Zhen-xia BAI Yuan-song LIN Yu-mei |
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| Institution: | . (Department of Hematology/Oncology, China-Japan Union Hospital of Jilin University, Changchun 130033, China ) |
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| Abstract: | Objective To investigate the gene transduction efficiency of lentiviral vector in CD34^＋ cord blood cells（CBCs） to provide key material for clinical gene therapy. Methods We used a third-generation self-inactivating （SIN） lentiviral vector system based on human immunodeficiency virus type 1 （HIV-1 ） to improve transduction efficiency. The cells were observed by flow cytometry （FCM） to detect GFP expression. Results Initial transduction efficiency was more than 95 % for the HIV vector. Conclusion This lentiviral vector is an excellent gene transduction system for CD34^＋ CBCs because of its high gene transduction efficiency. |
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| Keywords: | cord blood hematopoietic stem/progenitor cell GFP lentiviral vector gene transduction efficiency |
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