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低危人群中梅毒螺旋体抗体3种血清学检测方法应用研究
引用本文:王文武,杨杰,丛蓉,杨军,张真路.低危人群中梅毒螺旋体抗体3种血清学检测方法应用研究[J].中国实验诊断学,2009,13(9):1226-1228.
作者姓名:王文武  杨杰  丛蓉  杨军  张真路
作者单位:武汉亚洲心脏病医院检验中心,湖北,武汉430022
摘    要:目的探讨3种梅毒螺旋体血清学试验用于梅毒螺旋体感染的初筛和确认的必要性。方法对34933例标本采用酶联免疫吸附试验(EusA)进行梅毒螺旋体的初筛,初筛结果为可疑阳性的样本进行梅毒螺旋体明胶凝集试验(TPPA)和甲苯胺红不加热血清反应素试验(TRUST)的检测。结果559例标本初筛结果为可疑阳性,其中,TPPA阳性423例,TRUST阳性210例。结论3种检验方法的结果各有其价值,将3种方法的结果均报告给临床医生有助于临床医生的综合诊断,减少医患纠纷。

关 键 词:梅毒螺旋体  酶联免疫吸附试验  梅毒螺旋体明胶凝集试验  甲苯胺红不加热血清反应素试验

Clinical application of three tests for Treponema pallidum infection
Institution:WANG Wen-wu, YANG fie, CONG Rong, et al. ( Department of Clinical laboratory, Wuhan Asia Heart Hospital, Wuhan 430022, China)
Abstract:Objective To study the necessity of the screening and confirmation of Treponema pallidum infection by three serologic tests. Methods 34 933 serum samples were screened by ELISA method on Treponerna pallidum antibody. The samples with probable positive result were retested by toluidine red unheated serum test (TRUST) and Treponema pallidum particle assay (TPPA).Restilts There were 559 serum samples with probable positive result. There were 423 TPPA positive result samples and 210 TRUST positive result samples among the total. Conclusion To report the three results obtained from different methods which have their respective value to clinical doctors would be helpful for their synthetic diagnose and the decrease of medical fiction.
Keywords:treponema pallidum  ELISA  treponema pallidurn particle assay  toluidine red unheated serum test
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