| 藏灵菇发酵乳中乳酸菌的分离纯化与鉴定 |
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| 引用本文： | 黄颖,杜娜,张堃,郑永晨.藏灵菇发酵乳中乳酸菌的分离纯化与鉴定[J].中国实验诊断学,2010,14(4):517-519. |
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| 作者姓名： | 黄颖 杜娜 张堃 郑永晨 |
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| 作者单位： | 1. 吉林大学第二医院,中心实验室,吉林,长春,130041
2. 吉林大学中日联谊医院,手外科,吉林,长春,130033 |
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| 基金项目： | 吉林大学基本科研业务费项目 |
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| 摘 要： | 目的从藏灵菇发酵乳中分离益生乳酸菌,并研究其生理生化特性,为今后功能性乳制品的开发提供优良菌种。方法用乳酸菌分离培养基从藏灵菇发酵乳中分离乳酸菌;对分离的乳酸菌进行生理、生化鉴定和16S rRNA序列测定,并对其生长特性、耐酸及耐胆盐特性进行分析。结果从藏灵菇发酵乳中分离出两株乳酸菌LP1和LA1,初步鉴定两株乳酸菌分别为植物乳杆菌和嗜酸乳杆菌;并能抵抗pH 3.0的酸性环境和0.3%-0.5%的高胆盐环境。结论植物乳杆菌LP1和嗜酸乳杆菌LA1具有较好的耐酸性和胆盐耐受力,具备作为益生菌应用的潜能。
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| 关 键 词： | 藏灵菇 乳酸菌 分离 鉴定 |
Isolation and identification of probiotic strains from Tibet Kefir |
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| HUANG Ying,DU Na,ZHANG Kun,et al..Isolation and identification of probiotic strains from Tibet Kefir[J].Chinese Journal of Laboratory Diagnosis,2010,14(4):517-519. |
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| Authors: | HUANG Ying DU Na ZHANG Kun |
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| Institution: | HUANG Ying1,DU Na2,ZHANG Kun1,et al.(1.Central Laboratory,The Second Hospital of Jilin University,Changchun 130041,China,2.China-Japan Union Hospital of Jilin University) |
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| Abstract: | Objective To isolate Lactobacillus strains from Tibet Kefir,study their physiological and biochemical characteristics,and provide good bacteria strains for development of functional dairy products in the future.Methods Potentially probiotic Lactobacillus strains were isolated from Tibet kefir,then subjected to physiological and biochemical identification as well as 16S RNA sequencing,and tested for acid-and oxgall-resistance.Results Two Lactobacillus strains were isolated,which could grow in MRS medium and ... |
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| Keywords: | Tibet Kefir lactobacillus isolation identification |
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