Cordycepin protects PC12 cells against 6-hydroxydopamine induced neurotoxicity via its antioxidant properties |
| |
| Institution: | 1. School of Pharmacy, Jiangsu University, Zhenjiang, 212013, China;2. Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, 90112, Thailand;3. School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, China;1. Bio-IT Fusion Technology Research Institute, Pusan National University, Busan, Republic of Korea;2. Institute for Research & Industry Cooperation, Pusan National University, Busan, Republic of Korea;3. Department of Microbiology, Pusan National University, Busan, Republic of Korea;1. Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China;2. Department of Pharmacology, Jinan University, Guangzhou 510632, China;1. Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China;2. College of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China;1. State Key Laboratory of Quality Research of Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, China;2. Laboratory of Neurodegenerative Diseases, LKS Faculty of Medicine, and State Key Laboratory of Brain and Cognitive Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China;3. Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China;4. State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen, China |
| |
| Abstract: | Parkinson’s disease (PD) is a progressive neurodegenerative disorder that is characterized by degeneration and loss of dopaminergic neurons of the substantia nigra. Increasing evidence has indicated that oxidative stress plays a pivotal role in the pathogenesis of Parkinson’s disease (PD). Therapeutic options that target the antioxidant machinery may have potential in the treatment of PD. Cordycepin, a nucleoside isolated from Cordyceps species displayed potent antioxidant, anti-inflammatory and anticancer properties. However, its neuroprotective effect against 6-OHDA neurotoxicity as well as underlying mechanisms is still unclear. In this present study, we investigated the protective effect of cordycepin against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity and its underlying mechanism. We observed that cordycepin effectively inhibited 6-OHDA-induced cell death, apoptosis and mitochondrial dysfunction. Cordycepin also inhibited cell apoptosis induced by 6-OHDA as observed in the reduction of cytochrome c release from the mitochondrial as well as the inhibition of caspase-3. In addition cordycepin markedly reduced cellular malondialdehyde (MDA) content and intracellular reactive oxygen species (ROS) level. Cordycepin also significantly increased the antioxidant enzymes; superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in 6-OHDA-treated cells. The results obtained unambiguously demonstrated that cordycepin protects PC12 cells against 6-OHDA-induced neurotoxicity through its potent antioxidant activity. |
| |
| Keywords: | Cordycepin 6-hydrpxydopamine Parkinson’s disease PC12 cells Antioxidant |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
