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正常及帕金森病大鼠黑质细胞凋亡与左旋多巴剂量相关性的实验研究
引用本文:曹非,孙圣刚,王涛,陈吉相,刘昌勤,梅元武,曹学兵,童萼塘,骆芳.正常及帕金森病大鼠黑质细胞凋亡与左旋多巴剂量相关性的实验研究[J].中华物理医学杂志,2002,24(3):137-139.
作者姓名:曹非  孙圣刚  王涛  陈吉相  刘昌勤  梅元武  曹学兵  童萼塘  骆芳
作者单位:[1]华中科技大学同济医学院附属协和医院神经内科,武汉430022 [2]湖北省新华医院内科
基金项目:2 0 0 1年国家自然科学基金资助项目(基金编号 :30 1 70 334)
摘    要:目的 研究左旋多巴对黑质细胞的神经毒性作用。探讨合理应用左旋多巴治疗帕金森病的方法。方法 通过6-羟基多巴胺脑立体定向注射术制作大鼠帕金森病模型。采用TUNEL方法观察左旋多巴小(每天每公斤10mg),中(每天每公斤50mg),大(每天每公斤100mg)三种不同剂量,不同的作用时间(1d,3d,5d,7d)对帕金森病大鼠黑质细胞的毒性作用,并观察治疗后7d各项指标的变化。结果 帕金森病大鼠细胞凋亡数随着左旋多巴治疗的时间,剂量增加而增加。结论 左旋多巴能加速帕金森病大鼠黑质细胞凋亡,小剂量,间隔使用左旋多巴能有效减少其神经毒性作用。

关 键 词:细胞凋亡  剂量  相关性  实验研究  帕金森病  左旋多巴  黑质细胞  动物模型  神经毒性
修稿时间:2001年9月21日

The relationship between nigral cell apoptosis and different doses of L-dopa in normal rats and rats with Par kinson’s disease
CAO Fei ,SUN Shenggang,WANG Tao,CHEN Jixiang,LIU Changqin,MEI Yuanwu,CAO Xuebing,TONG Etang,LUO Fang.The relationship between nigral cell apoptosis and different doses of L-dopa in normal rats and rats with Par kinson’s disease[J].Chinese Journal of Physical Medicine and Rehabilitation,2002,24(3):137-139.
Authors:CAO Fei  SUN Shenggang  WANG Tao  CHEN Jixiang  LIU Changqin  MEI Yuanwu  CAO Xuebing  TONG Etang  LUO Fang
Institution:CAO Fei *,SUN Shenggang,WANG Tao,CHEN Jixiang,LIU Changqin,MEI Yuanwu,CAO Xuebing,TONG Etang,LUO Fang . * Department of Neurology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan
Abstract:Objective To evaluate the cytotoxic effects of levodopa on nigral cells and explore the method by which Parkinson's disease(PD) can be treated reasonably with levodopa. Methods One hundred 6-OHDA induced PD rats were assigned randomly into L-dopa group ( n =75) and PD group ( n =25). The rats in the former group were subdivided further into 3 subgroups (25 of each) and were treated respectively by intraperitoneal injections of Levodopa with different dosages (10 mg/kg/d, 50 mg/kg/d and 100 mg/kg/d). The cytotoxic effects on apoptosis of the nigral cells were observed at different durations( 1 d, 3 d, 5 d, 7 d) after the injections of levodopa. Parameters were compared among those groups, and 25 normal rats served as control. Results The number of substantia nigral apoptosis cells was increased and positively related to the changes of doses and durations of use of levodopa. Conclusion The Levodopa enhances the apoptosis of substantia nigra cells of PD rats, if it is given intermittantly and/or appropriate doses are used, its cytotoxic effect can be minimized.
Keywords:Parkinson's disease  Levodopa(L-dopa)  Apoptosis
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