次声作用对大鼠大脑皮层脂质过氧化的影响

目的：观察8Hz,130dB次声作用于大鼠不同时间后，大脑皮层脂质过氧化相关指标的变化，以探讨次声引起脑损伤的机理。方法：将35只Ⅱ级、雄性、SD大鼠随机分为对照组和次声作用7d,14d,21d,28d组五组，用8Hz,130dB次声作用，每天1次，每次2h，各组于最后一次作用结束后0．5－1h内取大脑皮层，制成10％的组织匀浆测定GSH－Px活性，MDA含量和SOD活性的变化。结果：与对照组相比，大鼠大脑皮层GSH－Px活性在7d组无显著性变化（P＞0．05），在14d组、21d组、28d组和显著性升高（P＜0．05）；MDA含量在7d组、14d组有显著怀升高（P＜0．05),21d组、28d组恢复至对照组水平（P＞0．05）；SOD活性有下降的趋势，但无显著性意义（P＞0．05）。结论：次声作用后，引发大鼠大脑皮层组织的脂质过氧化－抗氧化反应，造成脑组织的损伤。

Effects of infrasound on the pallial lipid peroxidation in rats

Objective To explore the effects of different times of exposure to infrasound on brain tissues in rats. Methods Thirty five male SD rats were equally and randomly divided into 5 groups:a control group, and four infrasound irradiation groups in which the rats were exposed to 8 Hz, 130 dB infrasound once a day and lasted 2 hours each time. Then, the levels of pallial glutathione peroxides(GSH-Px), malondialdehyde(MDA) and superoxide dismutase(SOD) of the irradiated rats were measured 9,14,21,and 28 days, respectively after the exposure. Results In the infrasound irradiation groups, the pallial GSH-Px content was more significantly increased at 14d ( P <0.05) than that in the control group, but there was no obvious difference before 7d ( P >0.05). The content of MDA was also increased more significantly before 14 d in the infrasound groups than that in the control group( P <0.05), but after 21d, it decreased to the level of the control group( P >0.05);The activity of SOD of the infrasound was not changed as compared with the control group( P >0.05). Conclusion Based on the findings, we can make the conclusion that 8 Hz 130 dB infrasound may induce the lipid peroxidation and anti peroxidation that damage the tissues and cells resulting in harmful effect on the brain tissues and cells.