康复训练对脑梗死大鼠血管内皮生长因子的表达及血管生成的影响

目的研究康复训练对脑梗死大鼠梗死灶周围新生血管情况、血管内皮生长因子（VEGF）和其受体（FLK-1）表达的影响。方法60只Wistar大鼠制备脑梗死模型后，随机分为康复训练组和造模对照组。康复训练组大鼠每天进行1h滚筒、平衡木、转棒及网屏训练，造模对照组置于普通笼中自由活动。每组分别于造模后3，7，14，21和28d采用免疫组织化学法检测梗死灶周围微血管数量（CD34标记）及VEGF和FLK-1的表达。结果（1）康复训练组术后14，21和28d行为学评分明显低于造模对照组（P〈0．05）；（2）康复训练组大鼠脑梗死灶周围CD34阳性微血管数量在脑梗死后21和28d较造模对照组明显增加（P〈0.05）；（3）康复训练组大鼠各时间点梗死灶周围VEGF表达均较对照组明显增加（P〈0．05）；（4）康复训练组大鼠各时间点梗死灶周围FLK-1表达均较造模对照组明显增加（P〈0．05）。结论康复训练可能通过诱导VEGF及其受体的表达，保护神经细胞，并促进血管新生，改善梗死灶周围血液供应，从而促进脑梗死后神经功能的恢复。

The effects of the rehabilitation training on vascularization and the expression of vascular endothelial growth factor after infarction

Objective To study the effects of rehabilitation training (RT) on vascularization and the expression of vascular endothelial growth factor ( VEGF) and its receptor, and on fetal liver kinase ( FLK-1 ) levels. Methods Infarct models were established through middle cerebral artery occlusion in sixty male Wistar rats, which were then randomly divided into a rehabilitation group and a control group. Rotating bar, balance beam and rolling cage exercises were employed with the rehabilitation group for 1 h every day, and the expression of CD34, VEGF and FLK-1 around the infarcts was detected using immunohistochemical methods at days 3,7, 14, 21 and 28 after infarction. Results ( 1 ) Compared with those in the control group, the behavior scores in the rehabilitation group were much lower at 14, 21 , and 28 days. (2)The number of CD34 microvessels around the infracts in the rehabilitation group was significantly greater than in the control group by the 21st and 28th days. (3) The expression of VEGF and FLK-1 around the infracts in the rehabilitation group was higher than that in the control group at all time points. Conclusion The expression of VEGF and FLK-1 around infracts can be improved through RT. It can promote recovery from central nervous system injury by developing neural protections and promoting vascularization after cerebral infarction.