灯盏花素抑制血管平滑肌细胞增殖的分子基础研究

Molecular basis study on inhibition of erigeron breviscapus element on the proliferation of vascular smooth muscle cell

Objective Applying cell transfection, RT-PCR Western blot, ELISA, ~3H-TDR and ~3H inositol unincorporation etc, to assay the thrombin receptor gene and protein expression, which was cultured by the fourth-sixth generation Sprague-Dawley rat (SD)'s vascular smooth muscle cell(VSMC) in vitro. To explore the influence of Erigeron breviscapus element and antisense thrombin receptor oligo-deoxy-nucleotides on the proliferation of VSMC. Results Erigeron breviscapus element and antisense thrombin can inhibit the proliferation of SD's VSMC, which is induced by thrombin. Comparing the obverse with control group, the statistic difference still exists(P<0.05) .The experimental result of RT-PCR, Western blot and ELISA indicates that Erigeron breviscapus element and antisense thrombin restrains the expression of mRNA and protein in SD's VSMC distinctly, and inhibits phosphoric inositol metabolism of Vascular Smooth Muscle Cell distinctly as well. There is no difference in inhabitation effects between Erigeron breviscapus element and atisense thrombin receptor. Methods Applying cell transfection, RT-PCR Western blot, ELISA, ~3H-TDR and ~3H inositol unincorporation etc, to assay the thrombin receptor gene and protein expression, which was cultured by the fourth-sixth generation Sprague-Dawley rat (SD)'s VSMC in vitro. To explore the influence of Erigeron breviscapus element and antisense thrombin receptor oligo-deoxy-nucleotides on the proliferation of VSMC. Results Erigeron breviscapus element and antisense thrombin can inhibit the proliferation of SD's VSMC, which is induced by thrombin. Comparing the obverse with control group, the statistic difference still exists(P<0.05). The experimental result of RT-PCR, Western blot and ELISA indicates that Erigeron breviscapus element and antisense thrombin restrains the expression of mRNA and protein in SD's VSMC distinctly, and inhibits phosphoric inositol metabolism of VSMC distinctly as well. There is no difference in inhabitation effects between Erigeron breviscapus element and atisense thrombin receptor. Conclusion Erigeron breviscapus element and antisense sequence of thrombin can inhibit the proliferation of SD's VSMC. The mechanism of action is fulfilled through inhibiting gene expression of thrombin receptor in order to suppress intracellular signal transmission. One of the action mechanisms of Erigeron breviscapus element on hypertensive cerebral hemorrhage is to suppress the proliferation of VSMC through blocking the expression of thrombin receptor which provide the theoretic and experimental basis to screen the drug action situs in cardio-cerebral disease with genetic technique.