| 改良酶学方法获得异种血管脱细胞支架 |
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| 引用本文： | 武欣,谷涌泉,段红永,陈兵,李建新,吴英锋,张淑文,汪忠镐,张建.改良酶学方法获得异种血管脱细胞支架[J].中国组织工程研究与临床康复,2011,15(47):8769-8772. |
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| 作者姓名： | 武欣 谷涌泉 段红永 陈兵 李建新 吴英锋 张淑文 汪忠镐 张建 |
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| 作者单位： | 首都医科大学宣武医院血管外科,北京市,100053 |
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| 基金项目： | 国家高技术研究发展计划(863 计划)资助项目，北京市自然科学基金资助项目，* |
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| 摘 要： | 背景:扩大脱细胞基质的孔径与孔隙率,保证其作为移植物的力学特性,是目前血管组织工程研究的热点之一。目的:观察改良的酶学方法处理异种血管的力学性能和组织相容性。方法:取猪的颈动脉作为基质,采用传统胰蛋白酶-EDTA加1%TritonX-100和0.1%氨水顺序脱细胞方案,并做了适当的方法改进,延长胰酶处理的时间,分别为胰酶处理4h,5h,6h。结果与结论:经组织学分析,脱细胞基质中无细胞成分,脱细胞的支架结构完整,随着胰酶处理时间的延长,其基质内弹力板破坏明显,孔径和孔隙率逐渐增大,缝合强度与爆破强度相对于自然血管虽略有降低,但差异无显著性意义。组织成分胶原蛋白含量也有所下降,尤其胰酶延长至6h组,其胶原蛋白含量明显低于正常未处理的自然血管(P<0.05)。结果显示延长胰蛋白酶处理时间后得到的脱细胞血管基质,具有良好的孔隙率、生物力学性能和组织相容性。
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| 关 键 词： | 脱细胞血管基质 胰酶 孔隙率 生物力学 生物相容性 支架 |
In vitro construction of decellularized vascular xenograft by improved enzymatic method |
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| Wu Xin,Gu Yong-quan,Duan Hong-yong,Chen Bing,Li Jian-xin,Wu Ying-feng,Zhang Shu-wen,Wang Zhong-gao,Zhang Jian.In vitro construction of decellularized vascular xenograft by improved enzymatic method[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(47):8769-8772. |
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| Authors: | Wu Xin Gu Yong-quan Duan Hong-yong Chen Bing Li Jian-xin Wu Ying-feng Zhang Shu-wen Wang Zhong-gao Zhang Jian |
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| Institution: | Wu Xin,Gu Yong-quan,Duan Hong-yong,Chen Bing,Li Jian-xin,Wu Ying-feng,Zhang Shu-wen,Wang Zhong-gao,Zhang Jian Department of Vascular Surgery,Xuanwu Hospital,Capital Medical University,Beijing 100053,China |
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| Abstract: | BACKGROUND: How to expand the pore size and porosity of the acellular matrix to facilitate the post-cell re-cultivation requirement, and simultaneously the graft must ensure its mechanical requirements, is one of the hotspots in vascular tissue engineering. OBJECTIVE: To observe the mechanical properties and histocompatibility of decellularized vascular xenograft vascular grafts constructed by the improved enzymatic method. METHODS: Porcine carotid arteries were decellularized using conventional trypsin-EDT... |
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