| 巴曲酶可影响人外周血内皮祖细胞定向分化及增殖能力 |
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| 引用本文： | 吴维,孙小杰,崔培元,鲁正. 巴曲酶可影响人外周血内皮祖细胞定向分化及增殖能力[J]. 中国组织工程研究与临床康复, 2011, 15(45): 8443-8446. DOI: 10.3969/j.issn.1673-8225.2011.45.018 |
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| 作者姓名： | 吴维 孙小杰 崔培元 鲁正 |
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| 作者单位： | 1. 蚌埠医学院第一附属医院肝胆外科,安徽省蚌埠市,233000
2. 安徽医科大学附属省立医院血管外科,安徽省合肥市,230001 |
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| 摘 要： | 背景:近年来内皮祖细胞促进机体血管新生方面的重要作用已形成共识,但内皮祖细胞的定向分化和扩增的问题是影响其疗效和广泛开展的主要原因。目的:观察人外周血内皮祖细胞体外分离、纯化、扩增和诱导分化为内皮细胞的可行性。方法:用密度梯度离心法从外周血获取单个核细胞,接种于包被有人纤维连接蛋白的细胞培养板中,培养6d后,收集贴壁细胞。以血管内皮细胞生长因子和碱性成纤维细胞生长因子对贴壁细胞进行诱导培养。另外,分别以0.05,0.1,0.2BU/mL巴曲酶培养贴壁细胞,观察内皮祖细胞的增殖能力。结果与结论:经血管内皮细胞生长因子和碱性成纤维细胞生长因子诱导后的细胞形态上表现内皮细胞特征,经流式细胞仪测定表达血管内皮细胞特有表面标志CD31和vWF,说明诱导后的细胞为内皮细胞。巴曲酶在体外培养条件下可增加血内皮祖细胞的数量,以0.1BU/mL浓度作用显著,且作用时间与血内皮祖细胞增殖能力的改善呈正相关。
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| 关 键 词： | 巴曲酶 外周血 内皮祖细胞 扩增 分化 |
Batroxobin influences the orientated differentiation and proliferation of endothelial progenitor cells from human peripheral blood in vitro |
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| Wu Wei,Sun Xiao-jie,Cui Pei-yuan,Lu Zheng. Batroxobin influences the orientated differentiation and proliferation of endothelial progenitor cells from human peripheral blood in vitro[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(45): 8443-8446. DOI: 10.3969/j.issn.1673-8225.2011.45.018 |
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| Authors: | Wu Wei Sun Xiao-jie Cui Pei-yuan Lu Zheng |
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| Affiliation: | Wu Wei1,Sun Xiao-jie2,Cui Pei-yuan1,Lu Zheng1 1Department of Hepatobiliary Surgery,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233000,Anhui Province,China,2Department of Vascular Surgery,Anhui Provincial Hospital of Anhui Medical University,Hefei 230001 |
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| Abstract: | BACKGROUND:In recent years,it has been known that endothelial progenitor cells(EPCs) promote the angiogenesis of the body,but the directed differentiation and proliferation of EPCs limits their efficacy and widespread application.OBJECTIVE:To investigate the feasibility of inducing human peripheral blood derived EPCs into endothelial cells in vitro,and to test their phenotype and function.METHODS:Mononuclear cells(MNCs) were isolated from the peripheral blood by using Percoll density gradient centrifugation... |
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