脐带结缔组织多潜能干细胞向内皮及心肌方向的分化

背景:脐带结缔组织富含多潜能干细胞,其免疫原性弱、增殖力强。目的:验证体外培养人类脐带结缔组织(wharton’s jelly)中多潜能干细胞向内皮细胞及心肌细胞方向的诱导分化。方法:贴块法培养人类脐带结缔组织多潜能干细胞。结果与结论:①流式细胞仪进行表型鉴定,多潜能干细胞CD105、CD73、CD90、CD44、CD54阳性,而CD45、CD34、CD106和CD133阴性。②加入血管内皮生长因子及碱性成纤维细胞诱导后,细胞表现内皮细胞形态,可以吞噬ac-LDL并结合UEA-1。③以5-氮杂胞甘诱导后经免疫组织化学染色显示,心肌细胞标记物肌钙蛋白Ⅰ和β肌球蛋白重链表达阳性。提示在体外培养的条件下,可以从脐带结缔组织中得到大量干细胞,该细胞可以向心肌及内皮方向分化。

Differentiation of multipotent stem cells derived from Wharton's Jelly into endothelial cells and myocardial cells

BACKGROUND: There are plenty of multipotent stem cells in Wharton’s Jelly that is a good stem cell resource. This kind of stem cell has the feasibility of low immunity and high proliferation. OBJECTIVE: To investigate the feasibility of multipotent stem cells derived from Wharton’s Jelly differentiating into endothelial cells and myocardial cells. METHODS: Multipotent stem cells were isolated from Wharton’s Jelly using the attached method. RESULTS AND CONCLUSION: Flow cytometer results showed that multipotent stem cells were positive for CD105, CD73, CD90, CD44, CD54, and negative for CD45, CD34, CD106 and CD133. Multipotent stem cells isolated from Wharton’s Jelly could swallow ac-LDL and bind UEA-1 after addition of vascular endothelial growth factor and basic fibroblast factor. After 5-azacytidine induction, troponin Ⅰ and β-myoglobulin were positive in myocardial cells. The findings indicate that a mass of stem cells can be obtained from Wharton’s Jelly, which can differentiate into endothelial cells and myocardial cells in vitro.