| SD大鼠许旺细胞提取及纯化:乳鼠和成年鼠的对照(英文) |
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| 引用本文： | 刘志新,宋宝辉,赵富生,李月珍,梁军.SD大鼠许旺细胞提取及纯化:乳鼠和成年鼠的对照(英文)[J].中国组织工程研究与临床康复,2010,14(6). |
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| 作者姓名： | 刘志新 宋宝辉 赵富生 李月珍 梁军 |
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| 作者单位： | 1. 牡丹江医学院组织学与胚胎学教研室,黑龙江省牡丹江市,157011
2. 牡丹江医学院原生物学教研室,黑龙江省牡丹江市,157011 |
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| 基金项目： | the Scientific Research Program of Mudanjiang Medical University,No.B200812~~ |
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| 摘 要： | 背景:许旺细胞是神经创伤修复的种子细胞,获取大量高纯度、高活性许旺细胞是研究的关键.目的:比较乳鼠和成年鼠许旺细胞的体外培养、纯化和形态学的差别,探讨简单可行的、可以获得高纯度许旺细胞的培养方法.方法:出生1～3 d SD大鼠20只和成年大鼠10只(体质量150～200 g),实验按细胞的来源分为新生组和成年组.双酶分步消化,二次接种差速分离纯化细胞;倒置显微镜观察细胞形态、贴壁速度;细胞计数,纯度计算;MTT法检测细胞增殖能力,绘制两组许旺细胞的增殖曲线,判定增殖速度;S-100免疫化学法鉴定细胞.结果与结论:乳鼠的许旺细胞贴壁速度快于成纤维细胞,成鼠的许旺细胞贴壁速度慢于成纤维细胞,两组许旺细胞纯度均达96%以上;MTT法检测两组许旺细胞增殖均活跃,由增殖曲线显示乳鼠许旺细胞增殖更快(P < 0.05);S-100免疫化学反应均呈阳性.提示双酶分步消化,二次接种差速分离纯化细胞,可以获取纯度高、活性良好的许旺细胞;乳鼠许旺细胞的增殖、贴壁能力更强.
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| 关 键 词： | 许旺细胞 细胞培养 坐骨神经 乳鼠 成年鼠 |
Extraction and purification of neonatal versus adult rat Schwann cells |
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| Liu Zhi-xin,Song Bao-hui,Zhao Fu-sheng,Li Yue-zhen,Liang Jun.Extraction and purification of neonatal versus adult rat Schwann cells[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2010,14(6). |
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| Authors: | Liu Zhi-xin Song Bao-hui Zhao Fu-sheng Li Yue-zhen Liang Jun |
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| Institution: | Liu Zhi-xin1,Song Bao-hui2,Zhao Fu-sheng1,Li Yue-zhen1,Liang Jun1 1Department of Histology , Embryology,2Department of Pathogeny Biology,Mudanjiang Medical University,Mudanjiang 157011,Heilongjiang Province,China |
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| Abstract: | BACKGROUND:Schwann cells are the seed cells of neural repair,and it is a key to harvest a large number of Schwann cells with high purity and activity.OBJECTIVE:To compare the in vitro culture,purification,and morphology of Schwann cells between neonatal and adult rats,and investigate a simple and feasible culture method to harvest high-purity Schwann cells.METHODS:Totally 30 Sprague-Dawley rats,comprising 20 neonatal (1-3 days after birth,neonatal group) and 10 adult (weighing 150-200 g,adult group) rats,we... |
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