骨形成蛋白4诱导成年大鼠骨髓间充质干细胞分化为神经元的能力

目的骨形成蛋白 4( bone morphogenetic protein 4,BMP4)体外诱导成年大鼠骨髓间充质干细胞分化为神经元的能力. 方法采用 BMP4体外定向诱导第五代骨髓间充质干细胞,免疫组化鉴定神经丝蛋白( NF)、胶质纤维酸性蛋白( GFAP)的表达. 结果成年大鼠骨髓间充质干细胞受 BMP4诱导后出现神经元样细胞 ,细胞免疫组化显示诱导出的神经元样细胞 NF表达阳性,诱导后 5 h, 12 h, 1 d, 3 d, 5 d和 7 d的 NF神经样细胞阳性率分别为 (40± 7)%, (71± 6)%, (58± 3)%, (53± 6)% , (37± 5)%, (13± 2)% ,诱导后 12 h,NF神经元样细胞阳性表达到高峰 ,与诱导后 5 h比较,差别有显著性 (t=1.380～ 2.621,P< 0.05). 结论 BMP4可以在体外诱导成年大鼠骨髓间充质分化为神经元.

Bone morphogenetic protein 4 induces the differentiation of adult rat bone marrow-derived mesenchymal stem cells into neurons

AIM:To evaluate the ability of bone morphogenetic protein 4 (BMP4) in inducing the in vitro differentiation of adult rat bone marrow-derived mesenchymal stem cells (MSCs) into neurons. METHODS:Using immunohistochemichal method,the expressions of neurofilament protein(NF) and glial fibriliary acidic protein (GFAP) were detected in BMP4-induced MSCs of the fifth passage. RESULTS:BMP4-induced MSCs were transformed into neuron-like cells,which were positive for NF expression as shown by immunocytochemical determination.The percentage of NF-positive neuron-like cells at 5 h,12 h,1 d, 3 d, 5 d and 7 d after induction was (40± 7)% ,(71± 6)% ,(58± 3)% ,(53± 6)% ,(37± 5)% and (13± 2)% respectively.The peak of NF expression in the neuron-like cells occurred at 12 h after induction,which was higher than that 5 h after induction(t=1.380- 2.621,P< 0.05). CONCLUSION:BMP4 can induce rat bone marrow-derived MSCs into neurons in vitro.