| 骨髓间充质干细胞抑制再生障碍性贫血患者外周血T细胞增殖的实验研究 |
| |
| 引用本文： | 刘增慧,肖扬,蒋祖军,李力,高飏,李勇华,李利,王耀春,邝丽萍.骨髓间充质干细胞抑制再生障碍性贫血患者外周血T细胞增殖的实验研究[J].中国组织工程研究与临床康复,2011,15(36):6679-6682. |
| |
| 作者姓名： | 刘增慧 肖扬 蒋祖军 李力 高飏 李勇华 李利 王耀春 邝丽萍 |
| |
| 作者单位： | 1. 解放军广州军区总医院血液科,广东省广州市,510010;广州中医药大学,广东省广州市,510405
2. 解放军广州军区总医院血液科,广东省广州市,510010 |
| |
| 摘 要： | 背景:既往研究发现骨髓间充质干细胞具有免疫抑制作用,而再生障碍性贫血患者存在T淋巴细胞异常活化及增殖。目的:体外分析骨髓间充质干细胞对再生障碍性贫血患者外周血T淋巴细胞增殖的抑制作用。方法:分离再生障碍性贫血患者外周血T淋巴细胞,行羧基荧光素乙酰乙酸琥珀酰亚胺酯(CFDASE)荧光染色,与体外培养扩增的骨髓间充质干细胞按3︰1比例直接接触法及Transwell法共培养,加入植物血凝素(PHA)刺激T淋巴细胞增殖,并设阴性及阳性对照。流式细胞术检测各组T细胞增殖情况,评价骨髓间充质干细胞对T淋巴细胞增殖的影响。结果与结论:CFDASE染色分析显示,直接接触组T细胞增殖较阳性对照组明显减低(P<0.01);Transwell组T细胞增殖亦较阳性对照组减低(P<0.05);直接接触组T细胞增殖较Transwell组明显减低(P<0.01)。骨髓间充质干细胞可能通过直接接触及分泌某些细胞因子方式抑制再生障碍性贫血患者异常增殖的T细胞,以直接接触方式发挥主要作用。
|
| 关 键 词： | 再生障碍性贫血 骨髓间充质干细胞 T淋巴细胞 增殖 免疫抑制 |
Experimental study on bone marrow mesenchymal stem cells inhibiting T lymphocytes proliferation in patients with aplastic anemia |
| |
| Liu Zeng-hui,Xiao Yang,Jiang Zu-jun,Li Li,Gao Yang,Li Yong-hua,Li Li,Wang Yao-chun,Kuang Li-ping.Experimental study on bone marrow mesenchymal stem cells inhibiting T lymphocytes proliferation in patients with aplastic anemia[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(36):6679-6682. |
| |
| Authors: | Liu Zeng-hui Xiao Yang Jiang Zu-jun Li Li Gao Yang Li Yong-hua Li Li Wang Yao-chun Kuang Li-ping |
| |
| Institution: | Liu Zeng-hui1,2,Xiao Yang1,Jiang Zu-jun1,Li Li1,Gao Yang1,Li Yong-hua1,Wang Yao-chun1,Kuang Li-ping1 1Department of Hematology,General Hospital of Guangzhou Military Command of Chinese PLA,Guangzhou 510010,Guangdong Province,China,2Guangzhou University of Chinese Medicine,Guangzhou 510405 |
| |
| Abstract: | BACKGROUND:Previous studies have found bone marrow mesenchymal stem cells(BMSCs) have immunosuppressive effects.T lymphocytes in patients with aplastic anemia(AA) are abnormal activatory and proliferous.OBJECTIVE:To study the inhibitory effects of BMSCs on T lymphocyte proliferation in vitro in AA patients.METHODS:T lymphocytes were isolated from the peripheral blood of patients with AA and stained with carboxyfluorescein diacetate succinimidyl ester(CFDA SE),and then co-cultured with BMSCs at 1:3 ratio usi... |
| |
| Keywords: | |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
