决明子水煎剂对营养性肥胖大鼠体质量增长抑制作用的量效关系

背景决明子具有降血压、降血脂、保肝及抑菌等活性,其减肥效应值得进一步探讨.目的观察大鼠生理状态下自然饮用决明子水煎剂对营养性肥胖大鼠体质量的影响.设计完全随机分组设计,对照实验,多组间比较采用方差分析和q检验.单位河南科技大学第二附属医院心血管病研究室.材料实验于2004-03/2004-09在河南科技大学第二附属医院心血管病研究室完成.选用雄性SD大鼠27只.将大鼠随机分为3组正常对照组、模型对照组和决明子组,每组9只.方法1]正常对照组喂基础饲料(蛋白质、脂肪、碳水化合物含量分别是18.2%,4.5%,55.2%,每克含14.54 kJ热量),自然饮水;模型对照组喂高营养性饲料(蛋白质、脂肪、碳水化合物含量分别是23.7%,21.6%,39.0%,每克含19.56 kJ热量),自然饮水;决明子组喂高营养性饲料,自然饮不同浓度的决明子水煎剂.决明子水煎剂的浓度从10 g/L(相当于每毫升含决明子生药10 mg)开始,每天增加一个百分比浓度(即10 g/L)直到第6天增至60g/L,从第7天开始保持60g/L的浓度不变直至实验第7周结束.2]每天定时记录进食量和饮水量,计算摄入热量(进食质量×每克所含热量),每周定时测体质量.第7周末,测量大鼠体长,计算Lee's指数3√体质量(g)×103/体长(cm)].3]多组间比较采用方差分析和q检验.主要观察指标决明子对营养性肥胖大鼠体质量、Lee,s指数、进食量、摄入热量和饮水量的影响.结果大鼠27只均进入结果分析.1]体质量实验第3～7周模型对照组的明显高于正常对照组(P＜0.05～0.01).实验第2～7周决明子组明显低于模型对照组(P＜0.05～0.01).2]Lee's指数实验第7周模型对照组和决明子组大鼠明显大于正常对照组(358.60±8.55),(341.84±7.29),(322.00±6.89)g/cm,P＜0.05～0.01],决明子组明显低于模型对照组(P＜0.05).3]进食量模型对照组和决明子组明显低于正常对照组(P＜0.05～0.01);决明子组与模型对照组相近(P＞0.05).4]摄入热量模型对照组和决明子组明显高于正常对照组(P＜0.05～0.01).决明子组与模型对照组相近(P＞0.05).5]饮水量决明子组基本与和模型对照组正常对照组相近(P＞0.05).模型对照组与正常对照组相近(P＞0.05).这表明60 g/L'质量浓度的决明子水煎剂不影响食欲.结论质量浓度60g/L的决明子水煎剂既能明显抑制营养性肥胖大鼠体质量的增加,且不影响食欲.

Dose-effect relationship of cassia seed decoction with its inhibition on increased body mass in nutritional obese rats

BACKGROUND: Cassia seed acts on decreasing blood pressure and blood lipid, protecting liver and inhibiting bacteria. It is worth to carry on a further discussion on its effect of weight loss.OBJECTIVE: To observe the influence of cassia seed decoction drunk naturally on body mass of nutritional obese rats in physiological state.DESIGN: Completely randomized grouping was designed, in which, control experiment, analysis of variance and q test were applied in comparison among groups.SETTING: Cardiovascular Institute, Second Affiliated Hospital, Henan University of Science and Technology.MATERIALS: The experiment was performed in Cardiovascular Instutute,Second Affiliated Hospital, Henan University of Science and Technology from March 2004 to September 2004, in which, 27 male SD rats were employed and randomized into 3 groups, named normal control group, model group and cassia seed group, 9 rats in each one.METHODS: 1] In normal control, the rats were bred with basic forage(the contents of protein, fat, carbohydrate were 18.2%, 4.5% and 55.2%successively, with 14.54 kJ caloric each gram) and drank water naturally.In model group, the rats were bred with high nutritive forage (the contents of protein, fat, carbohydrate were 23.7%, 21.6% and 39.0% successively,with 19.56 kJ caloric each gram) and drank water naturally. In cassia seed group, the rats were bred with high nutritive forage and drank cassia seed decoction of various concentration naturally. The concentration of cassia seed decoction started at 10 g/L (equally contained 10 mg raw cassia seed each milliliter) and was increased by 100% concentration each day (10 g/L)till to 60 g/L on the 6th day. Since the 7th day, the concentration of 60 g/L was maintained till to the 7th weekend. 2] It was to record appetite and drinking quantity at definite time every day and calculate absorbed caloric(intake mass × caloric contained each gram). It was to measure body mass at definite time each week. On the 7th weekend, the body length of rat was measured and Lee's index was calculated 3√body mass (g)×103/body length (cm)]MAIN OUTCOME MEASURES: Influences of cassia seed on body mass, Lee's index, appetite, caloric and drinking quantity in nutritional obese rats.RESULTS: Twenty-seven rats all entered result analysis. 1] Body mass:that in model group from the 3rd to 7th week in experiment group was higher remarkably than normal control group (P ＜ 0.05-0.01). That in cassia seed group from the 2nd to 7th week was lower remarkably than that in the model group (P ＜ 0.05-0.01). 2] Lee's index: that in model group and cassia seed group on the 7th week of experiment was higher remarkably than that in the normal control group (358.60±8.55), (341.84±7.29), (322.00±6.89) g/cm, P ＜ 0.05-0.01] and that in cassia seed group was lower remarkably than that in the model group (P ＜ 0.05). 3] Appetite: that in model group and cassia seed group was lower remarkably than that in the normal control group (P ＜ 0.05-0.01) and that in cassia seed group was near to the control group (P ＞ 0.05). 4] Absorbed caloric: that in model group and cassia seed group was higher remarkably than that in the normal control group (P ＜ 0.05-0.01) and that in cassia seed group was near to the control group (P ＞ 0.05). 5] Drinking quantity: that in cassia seed group was basically near to that in the model group and the control group (P ＞ 0.05) and that in model group was near to the control group. It was indicated that cassia seed decoction at mass concentration of 60 g/L did not affect appetite.CONCLUSION: Cassia seed decoction at mass concentration of 60 g/L inhibits remarkably the increased body mass of nutritional obese rats and is free from influence on appetite.