促胰素对大鼠胰岛细胞增殖及功能的影响

背景:有研究报道,促进胰岛β细胞增殖分化和再生是2型糖尿病患者一种潜在的治疗方案。目的:观察促胰素对体外培养大鼠胰岛细胞增殖及功能的影响。方法:采用胶原酶消化和组织培养法分离纯化大鼠胰岛细胞,检测其纯度和活性,将胰岛细胞分为空白对照组和实验组,空白对照组加入普通培养基,实验组培养基中加入不同质量浓度(100,200,300,400mg/L)促胰素,培养1,3,5d后CCK-8法检测细胞增殖活性;培养5d后行低糖和高糖刺激胰岛素释放实验。结果与结论:随着促胰素质量浓度的增高,胰岛细胞增殖活性呈剂量依赖性增加(P<0.05),但无明显时间依赖性。除100,200mg/L组外,300,400mg/L两组胰岛素分泌量均显著高于空白对照组(P<0.05)。表明300,400mg/L促胰素不仅可促进胰岛细胞增殖,而且可显著增强胰岛细胞分泌胰岛素的功能。

Effect of pancreatotrophin on the proliferation and function of islet cells in rats

BACKGROUND:Studies have reported to promote proliferation,differentiation and regeneration of β-cells is a potential treatment for patients with type 2 diabetes.OBJECTIVE:To investigate the effects of pancreatotrophin on the proliferation and function of rat islet cells in vitro.METHODS:The rat islet cells were isolated and purified by collagenase digestion and tissue-culture,followed with the detection of purity and activity.The islet cells were divided into blank control group and experimental group.Normal culture medium was added in the blank control group,pacreatotrophin in different concentrations were put in the experimental groups(100,200,300,400 mg/L respectively).CCK-8 detection was performed to test the proliferous activity after 1,3 and 5 days.After 5 days of culture,insulin release test was carried out in the condition of low-glucose and high-glucose.RESULTS AND CONCLUSION:Along with the increase of the pancreatotrophin concentration,the proliferate activity of islet cells was in a dose-dependent manner(P < 0.05),but it demonstrated that there was no obvious time-dependent;In addition to 100 mg/L group and 200 mg/L group,the volume of insulin secretion in 300 mg/L group and 400 mg/L group were significantly higher than that in the blank control group(P < 0.05).It demonstrates that the concentrations of pancreatotrophin in 300 mg/L and 400 mg/L can not only promote cell proliferation,but also can significantly enhance the function of islet cells to secrete insulin.