| 脑源性神经生长因子抗体对小鼠坐骨神经损伤后脊髓与背根节内Synaptophysin表达的影响 |
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| 引用本文： | 李昌琪,杨德森,罗学港,刘丹,伍校琼,卢大华. 脑源性神经生长因子抗体对小鼠坐骨神经损伤后脊髓与背根节内Synaptophysin表达的影响[J]. 中国组织工程研究与临床康复, 2002, 6(17): 2539-2540 |
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| 作者姓名： | 李昌琪 杨德森 罗学港 刘丹 伍校琼 卢大华 |
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| 作者单位： | 1. 中南大学湘雅医学院神经生物学研究室,湖南,长沙,410078
2. 中南大学湘雅二医院,湖南,长沙,410011 |
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| 基金项目： | 湖南省自然科学基金(98JJY2015) |
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| 摘 要： | 目的探查小鼠坐骨神经压榨损伤后内源性脑源性神经营养因子(brainderivedneuroliophicfactor,BDNF)对Synaptophysin(SYN)在相应脊髓前角运动细胞与背根节神经元内表达的影响。方法在小鼠一侧坐骨神经压榨损伤后腹腔注射BDNF抗体,中和内源性BDNF,然后用免疫组织化学方法观察SYN在与坐骨神经相连的脊髓前角运动细胞与背根节神经元的表达。结果实验组SYN免疫反应阳性神经元的数目较对照组明显减少,阳性细胞的平均光密度也显著下降(P<0.01)。结论小鼠坐骨神经压榨损伤后内源性BDNF可能参与脊髓前角运动细胞与背根节神经元内SYN的表达。
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| 关 键 词： | 脑源性神经营养因子 Synaptophysin 脊髓 背根节 坐骨神经 小鼠 |
| 文章编号： | 1671-5926(2002)17-2539-02 |
| 修稿时间： | 2002-06-26 |
The effects of brain derived n eurotr ophic factor antibody on the expression of Synaptophysin in the spinal co rd and dorsal root ganglion of mouse after sciatic nerv e lesion |
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| LI Chang-qi YANG De-sen,LUO Xue-gang,et al.. The effects of brain derived n eurotr ophic factor antibody on the expression of Synaptophysin in the spinal co rd and dorsal root ganglion of mouse after sciatic nerv e lesion[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2002, 6(17): 2539-2540 |
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| Authors: | LI Chang-qi YANG De-sen LUO Xue-gang et al. |
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| Affiliation: | LI Chang-qi YANG De-sen,LUO Xue-gang,et al.Xiangya Medical College,Central South Unive rsity,Changsha 410078,China |
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| Abstract: | Objective To investigate the eff ec t of endogen ous BDNF on regulating the expressio n of synaptophysin(SYN)in sp inal cord and dorsal root ganglion(DRG)of mouse after sciatic nerve crushed.M ethods BDNF antibody was injected intraperitoneal to neutralize the endogenous BDNF after sciatic nerve crushed,for one week survival,the S YN immunoreactivi ties were detecte d in the motor neuron of spinal cord and sensory neuron of DRG in corr esponding segment by immunohistoch emical technique.Result The number and the mean optical density of SYN-ir positive neuron we re decreased graduall y after sciatic nerve crushed followed by BDNF anti body injection.Conclusion T he results suggest endogenous BDNF may regulate the expression of SYN in spi nal cord and DRG after sciatic nerve i njured. |
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| Keywords: | brain derived neurotrophi c factor Synaptophysin spinal cord dorsal root ganglion sciatic nerve mouse |
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