巴曲酶复合低温对全脑缺血再灌注损伤的影响

目的观察低温、巴曲酶及巴曲酶复合低温对全脑缺血再灌注损伤的影响. 方法采用蒙古沙土鼠全脑缺血模型.观察缺血 10 min、再灌 20 min及 60 min时,应用巴曲酶、低温及巴曲酶复合低温对沙土鼠脑海马组织超氧化物歧化酶( superoxide dismutase,SOD)、丙二醛的影响. 结果随着缺血时间的延长 SOD活性有下降趋势,与对照组比较低温组复灌 20, 60 min分别下降 5.85%和 8.23%;巴曲酶组复灌 20, 60 min分别下降 18.3%和 5.12%.低温和巴曲酶均可减轻 SOD活性下降( P< 0.01);而巴曲酶复合低温组效果更优于单独应用( P < 0.05);丙二醛含量随着复灌时间的延长有增高趋势,能降低其生成含量( P< 0.01);两者复合应用效果更优. 结论巴曲酶及低温均具有脑保护作用,巴曲酶复合低温可增强脑保护作用.

Effects of batroxobin combined with hypothermia on complete cerebral ischemia-reperfusion injury

AIM:To explore the effects of hypothermia, batroxobin,hypothermia combined with batroxobin on complete cerebral ischemia-reperfusion injury. METHODS:Complete cerebral ischemia models of Menggu gerbil rats were employed.The effects of hypothermia, batroxobin,hypothermia and batroxobin on superoxide dismutase(SOD) and malondialdehyde(MDA) in gerbil hippocampus were observed under the condition of ischemia for 10 minutes and reperfusion for 20 and 60 minutes,respectively. RESULTS:Activity of SOD tended to decline with prolonged ischemia time, which decreased by 5.85% and 8.23% in the hypothermia group for 20 and 60 minutes of reperfusion,and 18.3% and 5.12% in batroxobin group for 20 and 60 minutes of reperfusion,respectively,compared with the control group.Both hypothermia and batroxobin can alleviate the reduction of SOD activity( P < 0.01) ;the combination of hypothermia and batroxobin was more effective than either used alone( P< 0.05) ;the content of MDA was increased with prolonged reperfusion time, this can reduce the production( P < 0.01) ;the combined application was much preferable. CONCLUSION:Both hypothermia and batroxobin have protective effects on brain, and combination of the two can enhance this effect.