重复经颅直流电刺激帕金森病模型大鼠的旋转行为

背景:经颅直流电刺激对帕金森病具有潜在的治疗作用,然而单次经颅直流电刺激的后效往往只能维持几个小时。目的:观察重复经颅直流电刺激对帕金森病大鼠旋转行为的治疗作用。方法:在SD大鼠黑质致密部和腹侧被盖区注射6-羟基多巴胺制作帕金森病大鼠模型,并完全随机分成阳极经颅直流电刺激组、阴极经颅直流电刺激组和对照组。对前两组大鼠初级运动区进行连续刺激10d,电流强度为80μA,刺激时间为30min/d的经颅直流电刺激。对照组不施加电刺激。结果与结论:重复阳极或阴极经颅直流电刺激对大鼠平均转速的减小存在显著的时间效应(P<0.05),刺激的后效可持续二三周;而对潜伏期和旋转持续时间改善作用不明显(P>0.05)。若保持两刺激组的刺激时间、刺激强度、刺激位置一致,则发现阴极经颅直流电刺激较阳极经颅直流电刺激对大鼠平均转速的减小更显著。结果提示使用重复经颅直流电刺激能够显著减小帕金森大鼠的旋转运动中的平均转速,且阴极刺激的效果更好。

Effects of repetitive transcranial direct current stimulation on praxiology of rats with Parkinson's disease

BACKGROUND:Although transcranial direct current stimulation(tDCS) has potential applications in clinical treatment of Parkinson’s disease,the aftereffects of single tDCS are limited in a few hours.OBJECTIVE:To investigate the efficacy of repetitive tDCS on rotational behavior of the rat models of Parkinson’s disease.METHODS:Substantia nigra pars compacta and ventral tegmental area of Sprague Dawley rats were injected 6-hydroxydopamine solution to construct Parkinson’s disease models and then were randomly divided into three groups:anodal tDCS,cathodal tDCS and control groups.The rats in the first two groups received single stimulation lasted for 10 consecutive days(current intensity:80 μA,stimulation time:30 min/d) at primary motor cortex,while in control group without any electrical stimulation.RESULTS AND CONCLUSION:Repetitive tDCS could lead to statistically significant difference of time in decreasing average speed(P < 0.05),and the aftereffects could maintain for 2-3 weeks,however,there was no significant changes in latency and duration(P > 0.05).Stimulation time,stimulation intensity and target location were kept the same between the two stimulation groups,the study showed that in the cathodal tDCS group average rotational speed decreased more significantly than that in the anodal tDCS group.It is indicated that repetitive rDCS can decrease the rotational speed of the rat models of Parkinson’s disease,and the effect of cathodal tDCS is better.