PCR与ELISA检测乙型肝炎病毒标志物的比较研究

目的探讨乙型肝炎病毒HBV-DNA与常见HBV血清免疫学标志物之间的相互关系及其临床检测意义。方法采用PCR法对HBV-DNA、ELISA法对HBV血清免疫学标志物同时进行检测，数据用SPSS11．0统计软件处理。结果899例11种常见模式HBV-DNA阳性率依次为90．48％、79．12％、73．91％、6667％、62，12％、30．68％、7．79％、7．69％、1．29％、8．54％、0．94％。HBV-DNA与HBsAg（P〈0，01）、HBeAg（P〈0．01）和抗HBc（P〈0．01）明显相关。结论HBV血清免疫学标志物、HBV-DNA的检测都很重要。HBsAg、HBeAg、抗HBc和HBV-DNA有很好的相关性和相似的流行病学意义，但两者不可互相替代。

Comparative study on HBV serum immune markers detected by PCR and ELISA

Objective To investigate the clinical significance and relationship between HBV-DNA and common HBV serum immune markers. Methods The HBV-DNA was detected using PCR technique and the HBV serum immune markers were detected using ELISA technique at the same time. The statistic data were processed with SPSS11.0. Results Positive infection rates of HBV-DNA in eleven common types of 899 patients were 90.48%, 79.12%, 73.91%, 66.67%, 62.12%, 30.68%, 7.79%, 7.69%, 1.29%, 8.54% and 0.94%. There were obvious correlativity between HBV-DNA and HBsAg (P<0.01) as well as HBeAg (P<0.01) and Anti-HBc (P<0.01).Conclusion It is necessary to detect HBV-DNA and HBV serum immune markers. HBsAg, HBeAg, Anti-HBc and HBV-DNA have strong relationship and similar epidemical significance, but they can't take place of each other.