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| 肺癌中P^53基因DNA水平突变与核蛋白 | |
| 引用本文： | 董光同,高元兴,胡永武,李红智.肺癌中P^53基因DNA水平突变与核蛋白[J].中国误诊学杂志,2003,3(3):326-328. |
| 作者姓名： | 董光同 高元兴 胡永武 李红智 |
| 作者单位： | 1. 浙江省温州医学院附属第一医院胸外科,325000 2. 浙江省温州医学院附属第一医院麻醉科,325000 3. 浙江省温州医学院附属第一医院遗传教研室,325000 |
| 基金项目： | 浙江省教委基金资助课题 (号 97- 1 57) |
| 摘 要： | 目的：研究肺癌P^53基因DNA水平和蛋白水平的突变。方法：应用PCR－SSCP银染技术检测了54例肺癌标本。结果：22例DNA水平突变阳性。突变率为41％，32例在核蛋白水平突变，突变率59％，其中22例DNA突变阳性，核蛋白突变均阳性。而32例DNA水平突变阴性，核蛋白突变阳性9例，占28％，P^53DNA突变与核蛋白的突变之间有显的相关性，并且两都与患的淋巴结转移有关（P＜0．01）。结论：PCR－SSCP是一种高灵敏快速检测P^53是否存在异常的有效方法，但是，P^53突变阳性患预后较差。 |
| 关 键 词： | 肺癌 P^53基因 核蛋白 联合梅链反应 点突变 |
| 文章编号： | 1009-6647(2003)03-0326-03 |
| 修稿时间： | 2002年11月25 |
Comparison between P~(53) Gene DNA Mutation and Neucleoprotein Mutation in Lung Cancer |
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| DONG Guang-tong,GAO Yuan-xing,HU Yong-wu,et al..Comparison between P~(53) Gene DNA Mutation and Neucleoprotein Mutation in Lung Cancer[J].Chinese Journal of Misdiagnostics,2003,3(3):326-328. | |
| Authors: | DONG Guang-tong GAO Yuan-xing HU Yong-wu |
| Abstract: | Objective To investigate the comparison between P 53 gene mutation and neucleoprotein mutation in lung cancer.Methods 54 samples of lung cancers were collected and PCR SSCP technique was used to detect the mutation.Results There were 22 DNA mutations (41%) and 32 neucleoprotein mutations (59%).In 22 DNA mutations,neucleoprotein mutations were also found,while in 32 no DNA mutations,9 neucleoprotein mutations were found(28%).P 53 gene DNA mutation showed significant correlation with neucleoprotein mutation and both correlated with lymph node metastasis(P<0.01).Conclusion PCR SSCP was a highly sensitive method to detect if P 53 gene mutated.Patients with P 53 mutations suffered poor prognosis. |
| Keywords: | Polymerase chain reaction P53 Gene P 53 Lung neoplasms/genetics Point mutation Nucleoproteins |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |