血管活性肠肽对胶原诱导关节炎大鼠治疗的作用初探

目的探讨血管活性肠肽(VIP)对胶原诱导性关节炎(CIA)大鼠的免疫调节作用,为应用VIP治疗类风湿关节炎(RA)提供理论依据。方法采用尾根部皮内多点注射法注射天然Ⅱ型胶原(CⅡ),免疫雌性Wistar大鼠(60只),建立CIA模型。随机取CIA成模大鼠(5只/组)于初次免疫后14~35 d,腹腔内注射不同浓度的VIP。定期进行临床、实验室、影像学及病理指标评估。结果不同剂量VIP(0.1、1、5 nmol隔日1次,共2周)治疗后CIA大鼠,其临床、实验室、影像学及病理指标明显缓解;正常鼠于VIP给药5 nmol 2周后重要脏器功能、组织学未见明显异常。结论生理浓度的VIP体内可缓解CIA鼠关节局部及全身免疫炎性反应,5 nmol 2周以内的体内治疗量基本安全,提示VIP可作为治疗RA有潜力的制剂。

NEUROIMMUNOREGULATING EFFECTS OF VASOACTIVE INTESTINAL PEPTIDE IN COLLAGEN INDUCED ARTHRITIS RATS

Objective To observe the neuroimmunoregulating effect of vasoactive intestinal peptide(VIP) in ameliorating collagen induced arthritis(CIA), providing an important implication for the clinic therapy of rheumatoid arthritis(RA) with VIP.Methods CIA models were established on 60 female Wistar rats by injection of Native Bovine type Ⅱ collagen(CⅡ) emulsified with complete Freund′s adjuvant.Clinical and laboratorial,histological,and radiological evaluations of CIA models were performed respectively.VIP was administered i.p.to CIA rats(5/group) at the specified doses(0.1,1,5 nmol) every other day from the 14th day(secondary immunization) to the 35th day after primary immunization.Results Clinical and laboratory severity of arthritis were significantly abrogated or ameliorated by treatment with VIP(0.1,1,5 nmol qod×2 week).No significant abnormality was caused by the administration of 5 nmol of VIP for two weeks.Conclusion 5 nmol of VIP could ameliorate inflammatory effects in CIA rats safely,suggesting VIP is a promising candidate for the treatment of RA.