MMP-2和TIMP-2在大肠癌组织中的表达及其意义

目的探讨基质金属蛋白酶(MMP-2)及其抑制物(TIMP-2)的表达与大肠癌浸润,转移的关系。方法应用免疫组织化学S-P法对58例大肠癌组织中MMP-2和TIMP-2进行检测,并以大肠腺瘤组织标本20例作为对照。结果MMP-2的表达率在大肠癌为74.1%,高于大肠腺癌瘤的35%(P<0.05)。其表达与大肠癌的浸润深度,Dukes分期和淋巴结转移均密切相关(P<0.05)。TIMP-2的表达在大肠腺瘤及大肠癌组织中无显著性差异(P>0.05),但其表达与大肠癌组织学类型。分化程度,淋巴结有无转移密切相关,大肠癌MMP-2和TIMP-2蛋白表达存在显著负相关(r=-0.3684,P<0.05)。结论MMP-2和TIMP-2对大肠癌浸润、转移均有明显影响。MMP-2蛋白阳性表达促进大肠癌浸润和转移,MMP-2和TIMP-2的检测可作为临床判断大肠癌的恶性程度、转移及估计预后的重要参考指标。

Expression and significance of MMP-2 and TIMP-2 in colorectal carcinoma

Objective To study the expression of matrix metalloproteinases-2(MMP-2) and tissue inhibitor of metalloproteinases-2(TIMP-2) in colorectal cancer tissues and the relation to the aggregated degree,metastasis and prognosis.Methods MMP-2 and TIMP-2 expressions were examined by S-P immunohistochemical method in 58 cases of colorectal cancer and 20 controls of the colorectal adenoma.Results The expression rate of MMP-2 was 35% in colorectal adenoma and 74.1% in colorectal cancer;the later had a close correlation with invasion depth,Dukes stages,metastasis of lymph node and prognosis(P<0.05).No significant difference in expression of TIMP-2 between colorectal adenoma and colorectal cancer(P>0.05),but there was significant relationship among TIMP-2 expression and histological type cellular differentiation and metastasis of lymph node,whereas its expression has no significant effect on prognosis(P>0.05).There was significant negative correlation between the expressions of MMP-2 and TIMP-2 in colorectal cancer(r=-0.3684,P<0.05).Conclusions The expressions of MMP-2 and TIMP-2 all have significant effects on the tumor biological behavior in colorectal cancer.To detect the expressions of MMP-2 and TIMP-2 may be helpful for judging tumor biological behavior and prognosis in colorectal carcinoma.