一氧化氮对急性肺损伤大鼠白细胞介素-6、环氧合酶-2的影响

目的探讨吸入一氧化氮(NO)对急性肺损伤大鼠肺组织白细胞介素-6(IL-6)和环氧合酶-2(COX-2)的影响。方法脂多糖(LPS)气管内滴注,制造大鼠急性肺损伤模型,吸入NO,检测肺组织中IL-6和COX-2的表达。结果LPS气管内滴注后,肺组织中COX-2以及IL-6的含量较对照组(A组)明显增加,吸入20 mg/L的NO后,肺组织中IL-6降低;吸入100mg/L的NO时,肺组织中IL-6、COX-2含量明显升高。结论吸入20 mg/L的NO,可以减轻肺损伤的程度,而吸入100 mg/L的NO,则使肺组织的IL-6、COX-2含量增加,加重肺损伤的程度。

Effect of nitric oxide on IL-6 and COX-2 in rats with acute lung injury

Objective To investigate the effect of inhaled nitric oxide (NO) on IL-6 and cox-2 in rat with acute lung injury(ALI) induced by lipopolysaccharide(LPS).Methods Twenty-four Sprague-Dawley rats were randomly divided into four groups: group N,normal control group;group L,LPS instilled intratracheally to induce ALI;group Z1,LPS + inhaled NO 20 mg/L and group Z2,LPS+ inhaled NO100 mg/L.Lung wet/dry weight ratios(W/D) were recorded to assess lung injury.The lung homogenates were prepared to detect IL-6 level by ELISA.The expression of COX-2 was detected by means of immunohistochemical staining.Results After LPS instilled,W/D ratio,IL-6 and COX-2 increased significantly.Compared with group L,W/D ratio decreased significantly in group Z1,but the ratio raised in group Z2;the level of IL-6 decreased significantly in group Z1;COX-2 level in group Z1 also decreased,but there was no statistical significant difference.Compared with group L,the level of IL-6 raised significantly in group Z2.Conclusion 20 mg/L NO inhalation decreased the level of IL-6 significantly,and the level of COX-2 also decreased.Low level of NO(20 mg/L) can down regulated inflammatory mediator expression in ALI rats.But high level NO(100 mg/L) might have deleterious effect on lung injury.