姜黄素联合顺铂对肺癌治疗的体外实验研究

目的探讨姜黄素(CUR)与化疗药(DDP)联合应用治疗肺癌的效应和可能机制。方法应用MTT试验检测CUR与DDP联用对人肺癌A549细胞增殖的影响,用流式细胞术检测CUR与DDP联用对人肺癌A549细胞周期和凋亡的影响。结果在一定的浓度范围内,随着姜黄素与顺铂均可抑制细胞生长,呈量-效关系。姜黄素与顺铂联用时,可以增强对A549细胞的增殖抑制作用。姜黄素和顺铂均可诱导A549细胞的凋亡,而且两者联用可增加A549细胞的凋亡率。姜黄素将细胞聚结在G2/M期并可诱导凋亡,顺铂将细胞聚结在S期并可诱导凋亡。结论姜黄素、顺铂均可抑制人肺腺癌A-549细胞的增殖、诱导细胞的凋亡,在一定浓度范围内,呈量-效关系,而且两者联合应用具有相加或协同作用。其效应可能是通过对细胞周期的影响来实现的。

In-Vitro Study of the Effect of Curcumin Combined with Cisplatin on Lung Cancer

Objective To study the effect and possible mechanism of curcumin(CUR),cisplatin(DDP) and their synergergistic effect on human lung adenocarcinoma cell line A549.MethodsThe absorbance of each cell was measured by MTT reduction assay.The growth inhibition ratio of A549 cells could be caculated.ResultsIn a certain concentration range,both CUR and DDP could inhibit A549 cell proliferation in a concentration-dependent pattern.Both CUR and DDP could significantly increase the apoptosis of A549 cells,and this action increased remarkably when DDP was combined with CUR.CUR made A549 cell show G2/M arrest,DDP made A549 cell show S arrest.ConclusionBoth CUR and DDP could enhance the growth inhibition and apotosis induction in a concentration-dependent pattern to human lung cancer cell.When they were combined,the effect could be enhanced in a synergistic or additive pattern.The possible mechanism may be cell cycle arrest.