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碱性磷酸酶对肌钙蛋白Ⅰ测定干扰的评价
引用本文:刘泽金,郑芳,张真路,范庆坤,王纯.碱性磷酸酶对肌钙蛋白Ⅰ测定干扰的评价[J].中华检验医学杂志,2010,33(9).
作者姓名:刘泽金  郑芳  张真路  范庆坤  王纯
作者单位:1. 武汉亚洲心脏病医院检验医学中心,430022
2. 武汉大学附属中南医院基因诊断中心
摘    要:目的 评价ALP对cTnI测定的干扰.方法 制备1份正常混合血浆和2份不同浓度cTnI的异常混合血浆,各分成8组,第1组为对照组,另7组加入不同浓度ALP,分别在ACCESS2 (Beckman Coulter公司)及AXSYM(美国Abbott公司)上测定每组标本的cTnI和ALP,并与对照组cTnI比较,评价ALP是否对cTnI测定产生干扰.结果 ACCESS cTnI测定正常血浆时,ALP浓度高达3 716 U/L时测定结果为(0.04±0.01)μgL,与正常血浆组的(0.04±0.01)μg/L比较差异无统计学意义(t=0.40,P>0.05);ALP高于917 U/L时,AXSYM cTnI测定结果为(0.08±0.01)μg/L,高于正常血浆组的(0.04±0.01)μg/L,差异有统计学意义(t=-4.89,P<0.01);ACCESS cTnI测定异常血浆时,ALP浓度高达3 534 U/L时,cTnI测定结果为(13.41±0.17)μg/L,与正常血浆组的(13.48±0.16)μg/L比较,差异无统计学意义(t=0.52,P>0.05).结论 高浓度ALP对ACCESS cTnI测定无干扰,不会导致假阳性结果,但高浓度ALP(917 U/L以上)会造成AXSYM cTnI检测干扰导致假阳性结果.

关 键 词:碱性磷酸酶  肌钙蛋白Ⅰ  假阳性反应

Interference of alkaline phosphatase on clinical cardiac troponin Ⅰ assay
LIU Ze-jin,ZHENG Fang,ZHANG Zhen-lu,FAN Qing-kun,WANG Chun.Interference of alkaline phosphatase on clinical cardiac troponin Ⅰ assay[J].Chinese Journal of Laboratory Medicine,2010,33(9).
Authors:LIU Ze-jin  ZHENG Fang  ZHANG Zhen-lu  FAN Qing-kun  WANG Chun
Abstract:Objective To evaluate the interference of ALP on cTnI assays. Methods One normal mixed plasma sample and 2 abnormal mixed plasma samples with different cTnI levels were prepared, and then divided them into 8 groups respectively. One group was randomly chosen as control while different amounts of ALP were added into the other seven groups. The concentrations of cTnI and ALP in each plasma portion were detected by ACCESS2 (Beckman-Coulter, Inc ) and AXSYM (Abbott Laboratories )separately. The results of the seven tested groups were then compared with those of the control, so as to evaluate whether ALP could interfere with the cTnI assay. Results When the chemiluminescent Access cTnI assay was carried out for detection of normal plasma, the concentration of ALP was up to 3 716 U/L and did not interfere with the test results of cTnI (0. 04 ±0.01) μg/L] compared with those of the control portion (0. 04 ± 0. 01 ) μg/L] (t = 0. 40, P > 0. 05 ). Once the concentration of ALP went beyond 917 U/L, the AXSYM cTnI assay results ( 0.08 ± 0. 01 ) μg/L] were higher than those of the normal control ( t =-4. 89, P<0. 01 ); When the concentration of ALP was up to 3 534 U/L, the test results of abnormal plasma cTnI detected by the Access assay ( 13.41 ±0. 17) μg/L] did not show significant differences from those of the control (13.48±0.16) μg/L] (t=0. 52,P>0.05).Conclusions High concentration ofALP did not interfere with the Access cTnI assay or lead to false positive results. However, the high level of ALP( > 917 U/L) could interfere with the AXSYM cTnI assay and cause a false positive result.
Keywords:Alkaline phosphatase  troponin Ⅰ  false positive reactions
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