首页 | 本学科首页   官方微博 | 高级检索  
检索        

上海地区人群血清骨转换标志物参考区间的建立
引用本文:宋斌斌,吴炯,张春燕,金杰,朱晶,郭玮,潘柏申.上海地区人群血清骨转换标志物参考区间的建立[J].中华检验医学杂志,2009,32(10).
作者姓名:宋斌斌  吴炯  张春燕  金杰  朱晶  郭玮  潘柏申
作者单位:复旦大学附属中山医院检验科,上海,200032
摘    要:目的 建立本实验室电化学发光法检测血清骨钙素(osteocalein,OCN)、Ⅰ型胶原羧基端肽β特殊序列(β-crossLaps,β-CTX)和总Ⅰ型前胶原氨基端肽(total procollagen type 1 amino-terminal propeptide,tP1NP)的参考区间.方法 根据临床和实验室标准化协会(CLSI)C48-A文件要求筛选出合适人群,按性别和绝经期前后分为男性、绝经前女性、绝经后女性3组.收集窄腹血清,使用Roche Modular E170电化学全自动免疫分析仪检测OCN、β-CTX、tP1NP.结果 393例合适人群中男性112名(年龄29~69岁)、绝经前女性148名(年龄28~54岁)、绝经后女性133名(年龄44~68岁),各组血清OCN、β-CTx、tP1NP的检测结果为男性(15.33±4.76)μg/L、(413±189)ng/L、(42.15±17.14)μg/L];绝经前女性(12.99±4.53)μg/L、265(30~820)ng/L、(36.43±14.23)μg/L];绝经后女性(18.96±5.15)μg/L、(513±195)ns/L、51.40(8.98~118.6)μg/L].除血清β-CTx(绝经前女性)和tP1NP(绝经后女性)各组数据经Log转换均为正态分布.各组的95%参考区间分别为:男性(6.00~24.66μg/L、43~783 ns/L、9.06~76.24μg/L);绝经前女性(4.11~21.87μg/L、68~680 ns/L、8.53~64.32μg/L);绝经后女性(8.87~29.05μg/L、131~900 ns/L、21.32~112.8μg/L).结论 本实验室建立的3项血清骨转换标志物的参考区间与厂商提供的有差异,各实验室在引用时应加以注意.

关 键 词:骨钙素  胶原  肽碎片  生物学标记  参考值

Serum bone turnover markers reference intervals establishment in population of Shanghai
SONG Bin-bin,WU Jiong,ZHANG Chun-yan,Zha ring,JIN Jie,GUO Wei,PAN Bai-shen.Serum bone turnover markers reference intervals establishment in population of Shanghai[J].Chinese Journal of Laboratory Medicine,2009,32(10).
Authors:SONG Bin-bin  WU Jiong  ZHANG Chun-yan  Zha ring  JIN Jie  GUO Wei  PAN Bai-shen
Abstract:Objective To establish the reference intervals of serum osteocalcin (OCN), C-terminal cross-linking telopeptide of type Ⅰ collagen (β-CTx) and total type Ⅰ procollagen N-terminal peptide (P1NP) by electrochemiluminescence assay. Methods According to the Clinical and Laboratory Standards Institute (CLSI) "CA8-A" document the appropriately healthy people, who were divided into three groups (men, premenopausal women, and postmenopausal women) by sex and pre- or postmenopausal status were screened. The levels of fasting serum of OCN,β-CTx, tPINP were detected by Roche Modular E170 electrochemical immunoassay. Results 393 appropriately healthy people consists of 112 men between the ages of 29 and 69 years, 148 premenopausal women between the ages of 29 and 69 years, 133 postmenopausal women between the ages of 29 and 69 years. The levels of serum OCN, β-CTx, tP1NP in men group were (15.33±4.76) μg/L, (413±189) ng/L, (42.15±17.14) μg/L, respectively. The levels of serum OCN, β-CTx, tP1NP in premenopausal women group were (12.99±4.53) μg/L, 265(30-820) ng/L, (36.43±14.23) μg/L, respectively. The levels of serum OCN, β-CTx, tP1NP in postmenopausal women group were (18.96±5.15) μg/L, (513±195) ng/L, 51.40 (8.98 -118.6)μg/L, respectively. Logarithmic transformation produced normal distributions for all markers but serum β-CTx of premenopausal women group and serum tPINP of postmenopausal women group. The 95% of the distribution intervals for serum OCN, β-CTx, tP1NP in men group was 6.00-24.66 μg/L, 43-783 ng/L, 9.06-76.24 μg/L, respectively. The 95% of the distribution intervals for serum OCN, β-CTx, tP1NP in premenopausal women group was 4.11-21.87 μg/L, 68-680 ng/L, 8.53-64.32 μg/L respectively. The 95% of the distribution intervals for serum OCN, β-CTx, tPl NP in postmenopausal women group were 8.87-29.05 βg/L, 131-900 ng/L, 21.32-112. 80 μg/L, respectively. Conclusions Compared with the reference intervals provided by manufacture, the reference intervals of three serum bone turnover markers established by our laboratory have great difference. Laboratory should pay attention to the reference intervals was cited.
Keywords:Osteocalcin  Collagen  Peptide fragments  Biological markers  Reference values
本文献已被 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号