Assessment of apheresis platelets during 5 days of storage: A National Cancer Institute,Cairo University experience |
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| Institution: | 1. Clinical Pathology Department, National Cancer Institute, Cairo University, Cairo, Egypt;2. Biostatistics and Cancer Epidemiology Department, National Cancer Institute, Cairo University, Cairo, Egypt;3. Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Egypt;1. Fundação Pró-Sangue - Hemocentro de São Paulo, São Paulo, SP, Brazil;2. Disciplina de Hematologia, Hemoterapia e Terapia Celular da Faculdade de Medicina da Universidade de São Paulo, São Paulo, SP, Brazil;3. Disciplina de Ciências Médicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil;4. Department of Obstetrics and Gynecology, Weill Cornell Medicine, New York, USA;5. Institute of Tropical Medicine, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil;6. Instituto da Criança do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil;7. Instituto do Tratamento do Câncer Infantil, São Paulo, Brazil;8. Laboratório de Investigação Médica 31 (LIM-31) – Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil;1. Axencia Galega de Sangue, Órganos e Tecidos (ADOS), Santiago de Compostela (A Coruña), Spain;2. Universitat Autònoma de Barcelona, Spain;3. Hospital Clínico Universitario, Santiago de Compostela (A Coruña), Spain;1. Department of Pharmacy, University of North Carolina Medical Center, Chapel Hill, NC, United States;2. UNC Eshelman School of Pharmacy, Chapel Hill, NC, United States;3. University of Washington Medical Center, Seattle, WA, United States;4. Lineberger Comprehensive Cancer Center, Chapel Hill, NC, United States;1. Surat Raktadan Kendra & Research Centre, Surat, Gujarat, India;2. National Institute of Immunohematology (NIIH, Mumbai), Mumbai, Maharashtra, India |
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| Abstract: | BackgroundPlatelet transfusion therapy is widely used to prevent hemorrhage in patients with thrombocytopenia and platelet disorders. The platelet concentrate (PC) quality is affected by increased storage time, as reflected in the decreased number of platelets, morphological changes, and impaired functions. This study aimed to analyze the impact of 5 days storage on platelets count and the expression of CD63, and Annexin V as activation markers during PC storage.MethodsFifty PCs collected from single donors were tested for platelet count on days 0, 3, and 5 using a Sysmex blood counter. CD61, CD63, and Annexin V expression was analyzed by a multicolor Navios flow cytometer.ResultsThere was a significant decrease in platelet count during 5 days of storage. There was a direct relationship between storage time and degree of platelet activation. CD63 had almost double increased expression on day 5 than day 3. Annexin V showed significantly increased expression on day 3 with minor differences between days 3 and 5.ConclusionAccording to standard blood bank conditions, PC stored for 5 days showed a degree of in vitro activation as evidenced by CD63 and Annexin V expression, may lead to reduced therapeutic efficacy. Flow cytometry monitoring platelet activation in PC offers a better understanding of the changes during PC storage and may help improve platelet products. |
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| Keywords: | Platelet transfusion CD63 Annexin V |
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