Serology and gene sequence analysis of the B(A) subtype of patients in Hunan |
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| Institution: | 1. Transfusion Medicine & Blood Centre, Sarvodaya Hospital & Research Centre, Faridabad, Delhi NCR, India;2. Medical Oncology & Hematology, Sarvodaya Hospital & Research Centre, Faridabad, Delhi NCR, India;3. Medical Administration, Sarvodaya Hospital & Research Centre, Faridabad, Delhi NCR, India;1. Department of Transfusion Medicine, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Homi Bhabha National Institute, Navi Mumbai 410210, India;1. Servizio di Immunoematologia e Medicina Trasfusionale, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy;2. Bone Marrow Transplantation, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy;1. Department of Transfusion Medicine, All India Institute of Medical Sciences, Rishikesh, India;2. Department of Neonatology, All India Institute of Medical Sciences, Rishikesh, India;3. Department of Transfusion Medicine, All India Institute of Medical Sciences, Jodhpur, India |
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| Abstract: | IntroductionB(A) is a relatively rare ABO subtype. The frequency of B(A) in European Caucasians is low, but is higher in China. B(A) is common in northern China, gradually decreasing from north to south. The frequency of B(A) has been researched in northern China, but not in southern China. This study aimed to investigate the serological characteristics and molecular mechanism of the B(A) subtype in Hunan.MethodsThe phenotypes of ABO blood group were analyzed by blood group serology for the patients in the Third Xiangya Hospital from 2016 to 2020; Exons 1–7 of the ABO gene were screened by PCR-SSP and sequencing.ResultsNine cases were suspected as belonging to the B(A) subtype by serological tests. The activity of D-galactosyltransferase in these patients' serum was significantly higher than that of group B plasma; meanwhile, there was no activity of Nacetylgalactosyltransferase. The ABO genotype was ABO*B.01/O.01.01 or ABO*B.01/O.01.02, and the gene sequencing results confirmed the phenotype as B(A), whose gene frequence was 1/26,000. Allele ABO*BA.02 (1/38,000) had the highest frequency, followed by ABO*BA.04 (1/77,000).ConclusionAmong the patients in Hunan, the most common allele encoding the B(A) phenotype was ABO*BA.02. The identification of the B(A) blood group required serological methods combined with genotyping. |
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| Keywords: | B(A) Blood group serology Gene sequence analysis Patients in Hunan |
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