结核分泌蛋白MPT64用于101例结核病快速诊断的研究

目的：比较痰菌检查、结明试验、PPD实验和MPT64蛋白抗体检测，评价MPT64蛋白在结核病血清学诊断中的价值。方法：对101例结核病患者进行分析，同时进行痰菌检查、结明试验、PPD实验和MPT64蛋白抗体检测，对结果进行比较对照分析。结果：痰检阳性率20．8％0，结明试验45．5%，PPD试验77．2％，PPD抗体79．2%，MPT64抗体74．2％；在肺结核患者，MPT64抗体与PPD试验一致率为75．6％；在胸膜炎患者，MPT抗体检测特异性高达86．7％。结论：PPD试验仍是目前结核病早期诊断与鉴别诊断的重要方法，分泌蛋白MPT64可作为新型结核病快速诊断试剂的组分。

Study on secreted protein MPT64 of mycobacterium tuberculosis for rapid diagnosis

Objective To compare sputum smear, MycoDot TM, PPD and anti-MPT64 antibody assay in tuberculosis patients and evaluate the value of MPT64 protein in serodiagnosis of tuberculosis. Methods Smear, MycoDot TM, PPD and anti-MPT64 antibody assay were used to analyze 101 cases of tuberculosis at equal pace and the results were analyzed statistically. Results The positive rate of smear assay was 20.8%, MycoDot TM assay was 45.5%, PPD test was 77.2%, anti- MPT64 antibody was 74.2%. The concordance rate of anti-MPT64 antibody assay to PPD assay was 75.6% in pulmonary tuberculosis patients. And in tuberculosis pleurisy patients, the specificity of anti-MPT64 antibody assay reached to 86.7%. Conclusion The PPD assay is still the main method for the diagnosis and secreted protein MPT64 may be the composition of new reagent for the rapid diagnosis of tuberculosis.