百令对慢性肾衰竭大鼠模型肾纤维化治疗机制研究

目的观察百令对慢性肾衰竭大鼠模型24h尿蛋白定量、血肌酐、肾脏组织病理及细胞因子表达的影响，探讨其对肾纤维化的治疗作用。方法80只SD大鼠随机分为假手术组（20只）和手术组（60只），手术组行5／6。肾切除术，模型制作成功后分为手术未治疗组15只、百令低剂量组（1．0g／（kg·d））15只、百令中剂量组（2．0g／（kg·d））15只和百令高剂量组（3．og／（kg·d））15只。假手术组随机分为假手术组未治疗组10只和假手术百令组（2．0g／（kg·d））10只。假手术百令组和百令低、中、高剂量组于手术4周后开始给药，每日灌胃，假手术组未治疗组、手术未治疗组每日生理盐水灌胃。治疗4周后检测24h尿蛋白定量、血肌酐，取残肾检测肾小球系膜基质面积／肾小球囊面积及肾小管-间质损伤指数，免疫组织化学法检测肾组织转化生长因子-β1表达。结果手术组24h尿蛋白定量、血肌酐、肾小球系膜基质面积／肾小球囊面积及肾小管-间质损伤指数、肾组织表达转化生长因子-β1较假手术组升高（P〈0．01），百令各治疗组上述指标较手术未治疗组下降（P〈0．01），百令高剂量组较中、低剂量组上述指标下降，两两比较差异均有统计学意义（P〈0．01）。结论百令可保护残肾功能，通过下调转化生长因子-β1表达延缓肾纤维化进展。

Therapeutic effect of Bailing on renal fibrosis in rat models with chronic renal failure

Objective To explore the therapeutic effect of Bailing on renal fibrosis by observing 24-hour urinary protein excretion, serum creatinine, histopathologic features and cytokine expression in rat models with chronic renal failure. Methods Eighty SD rats were randomly divided into sham operation group （n = 20） and operation group （n = 60）. Operation group underwent 5/6 nephrectomy to establish rat models and was redivided into operation untreated group, low-dose Bailing group （1. 0 g/（kg·d））, medium-dose Bailing group （2. 0 g/（kg · d）） and high-dose Bailing group （3.0 g/（kg· d））, with 15 rats in each group. Sham operation group was randomly redivided into sham operation untreated group and sham Bailing group （2.0 g/（kg · d））, with 10 rats in each group. Bailing groups received lavage with Bailing everyday 4 weeks after operation. Untreated groups received lavage with normal saline. The 24-hour urinary protein excretion and serum creatinine were detected. Mesangial matrix area/glomerular capsule area and renal tubule- mesenchymal injury indexes were measured. The expression of transforming growth factor-β1 （TGF-β1） was determined by immunohistochemical method. Results The 24-hour urinary protein excretion, serum creatinine, mesangial matrix area/glomerular capsule area, renal tubule-mesenchymal injury index and TGF-β1 were significantly higher in operation group than those in sham operation group （P〈0.01）, were lower in Bailing groups than those in operation untreated group （P〈0.01）, and were lower in high-dose Bailing group than those in medium- and low-dose Bailing groups, showing significant differences in multiple comparison （P〈0.01）. Conclusions Bailing could protect the remnant kidney function and postpone renal fibrosis by decreasing the expression of TGF-β1 in renal tissues.