荧光定量PCR观察中药抗病毒胶囊对豚鼠HSV-2系统感染的影响

目的探讨中药抗病毒胶囊治疗生殖器疱疹的作用机理。方法用抗病毒胶囊治疗初发性生殖器疱疹豚鼠模型,然后对豚鼠的大脑、脊髓、宫颈、阴道进行HSV鄄2DNA荧光定量PCR检测。结果在各组动物中,4个不同部位均有病毒DNA的存在;不同部位的病毒拷贝量有差异,不同组之间相应部位比较也存在差异。经单因素方差分析,脊髓、阴道处病毒拷贝量最高,大脑、宫颈处病毒拷贝量相当;生理盐水组与其它各组比较相应部位差异均有显著性(P<0.01)。中药抗病毒胶囊组与阿昔洛韦组各处病毒拷贝量差异无显著性(P>0.05)。结论中药抗病毒胶囊治疗生殖器疱疹的作用机理可能为阻止HSV的潜伏和抑制HSV的复制增殖。

The Effect of Chinese Herbal Capsule on Experimental Genital Herpes

Objective To study the mechanism of Chinese herbal capsule in the treatment of genital herpes.Methods Guinea pig model for genital herpes infection was established.HSV DNA from the cerebrum,spinal cord,cervical canal and vagina of guinea pigs was detected by fluorescent-quantitative PCR.Results The HSV DNA could be detected from all four kinds of tissues,with different copies of the virus in the different tissues,the highest in spinal cord,then in vagina,the least in cerebrum and cervical canal.There was statistical difference of HSV-DNA copies between herbal capsule group and control group(normal saline),whereas no statistical difference between herbal capsule group and aciclovir group.Conclusion The mechanism of the anti-virus herbal capsule in the treatment of experimental genital herpes might be related to blocking the latent infection and suppressing the replication of HSV.