止血环酸对紫外线诱导的色素斑黑素细胞的影响

摘要 目的：研究止血环酸皮内注射对黄褐斑皮损黑素细胞的影响，为外用止血环酸治疗黄褐斑提供实验依据。方法：采用紫外线照射豚鼠裸露皮肤制作黄褐斑动物模型，随机分为实验组和对照组，实验组黄褐斑皮损内注射止血环酸。通过免疫组化方法检测HMB45表达阳性的黑素细胞，Masson Fontanna-Ponceau染色检测黑素含量，图像分析用药前后黑素细胞和黑素的变化。结果：止血环酸注射后较注射前，黄褐斑皮损内黑素细胞无明显差异（P>0.05），黑素明显减少，差异有统计学意义（P<0.05）。结论：皮内注射止血环酸对黑素细胞无明显影响，但可以抑制黑素生成。

Effect of tranexamic acid on melanocytes in pigmented patches of mice induced by ultraviolet rays

Abstract Object:To investigate the effect of intradermal microinjection of Tranexamic Acid on Melasma’s melanocytes and melanin, to provide experimental evidence for treating Melasma. Methods: To establish Melasma Models with Guinea pig by UVB. Then, these models were divided into experimental group and control group. The pigmental spot of experimental group were intradermal microinjected with Tranexamic Acid. The change of number of melanocytes and melanin granules in both groups were observed in the immunohistology and Masson Fontanna-Ponceau staining.Result: The change of melanocyte in Melasma pigmental spot which microinjected with Tranexamic Acid, was not significant compared with before injection（P>0.05）. But the contents of melanin was obvious decrease（P<0.05）.Conclusion:There are inhibitory effect of Tranexamic Acid on the melanin granules,but no effec on melanocytes.