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高通量测序分析重度寻常痤疮患者和健康人肠道菌群差异
引用本文:闫慧敏,赵惠娟,郭独一,张春雷,姜薇.高通量测序分析重度寻常痤疮患者和健康人肠道菌群差异[J].中华皮肤科杂志,2017(3):172-176.
作者姓名:闫慧敏  赵惠娟  郭独一  张春雷  姜薇
作者单位:100191,北京大学第三医院皮肤科
基金项目:国家自然科学基金(81201216)National Natural Science Foundation of China (81201216)
摘    要:目的 利用高通量测序技术分析重度寻常痤疮患者和健康人肠道菌群的差异.方法 门诊确诊的重度寻常痤疮患者10例及年龄、性别相匹配的健康人10例,分别收集粪便标本,提取细菌DNA,行16S rRNA测序鉴定微生物的种类,并进行菌群差异分析.结果 两组肠道菌群多样性未发现显著差异,仅在肠道菌群的相对丰度上发现个别具有显著差异的菌.两组标本主要由拟杆菌门、厚壁菌门、变形菌门、放线菌门组成.比较两组在门、属水平上菌群相对丰度,差异均无统计学意义;在种水平上,Blautia producta、Coprococcus eutactus在两组间差异有统计学意义.结论 重度寻常痤疮患者和健康人相比,菌群多样性指数未见明显差异.但某些细菌菌种存在显著差异.

关 键 词:寻常痤疮  肠道菌群  高通量测序

High-throughput sequencing analysis of gut microbiome in patients with severe acne vulgaris and healthy individuals
Yan Huimin,Zhao Huijuan,Guo Duyi,Zhang Chunlei,Jiang Wei.High-throughput sequencing analysis of gut microbiome in patients with severe acne vulgaris and healthy individuals[J].Chinese Journal of Dermatology,2017(3):172-176.
Authors:Yan Huimin  Zhao Huijuan  Guo Duyi  Zhang Chunlei  Jiang Wei
Abstract:Objective To compare differences of gut microbiome between patients with severe acne vulgaris and healthy individuals by using high-throughput sequencing technology.Methods Stool samples were collected from 10 outpatients with severe acne vulgaris and 10 age-and sex-matched healthy controls.Then,the bacterial DNA was extracted and subjected to 16S rRNA sequencing for the identification of microbial species,and the differences of gut microbiome were compared between the patients and controls.Results There were no significant differences in the diversity of intestinal microflora,but only the relative abundance of a few bacteria differed significantly between the two groups.Gut microbiome in the two groups mainly consisted of Bacteroidetes,Firmicutes,Proteobacteria and Actinobacteria.There were no significant differences in the relative abundance of bacteria at the phylum and genus levels between the two groups.However,the relative abundance of Blautia producta and Coprococcus eutactus at the species level differed remarkably between the two groups.Conclusion No significant differences in the bacterial diversity indices are found,but some bacterial species significantly differ between the patients with severe acne vulgaris and healthy controls.
Keywords:Acne vulgaris  Gut microbiome  High-throughput sequencing
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