生殖道分泌物支原体培养、半定量与耐药率调查

近年来,泌尿生殖道支原体感染,越发引起重视。检出手段也不断创新,新药不断开发,而支原体的耐药谱也不断发生变化,为此,要了解目前北京地区泌尿生殖道支原体中解脲支原体(Uu)和人型支原体(Mh)的感染率和耐药性,笔者选用了IST与DM培养试剂盒,对170例生殖道标本进行了培养检测,又将前次培养阳性结果与耐药率一并统计,临床分析另文报道,现就实验部分报告如下:

Culture, isolation rate and drug resistance rate of mycoplasma from genital tract secretions

Objective Culture,colony count,and sensitivity and resistance to ten antibiotics of mycoplasma from secretions of genital tract were investigated,and two assay kits(home made and imported)were com- pared.Methods A French made assay kit developed by bioMerieux Co.(Mycoplasma IST)and a Chinese made assay kit developed by Ganquan Hospital(DM)were used,both of which serve the integrated purposes of iden- tification,colony count and drug sensitivity.Results The coincidence rate of IST and DM was 86.79%,with a high correlation(X~2=24.63,P<0. 005).The total isolation rate of mycoplasma was 55.88%,in which the isola- tion rates of ureaplasma urealyticum(Uu)and mycoplasma hominis(Mh)were 38.24% and 17.65%,respec- tively.The total isolation rate in males was 52.41% while that in females was 76.0%.The isolation rates from semen,prostatic secretion and cervical mucus were 56.14%,38.71% and 76.0%,respectively.The resistance rates of 106 strains of Uu to minocycline,spectinomycin,azithromycin,sparfloxacin,pristinamycin, josamycin,doxycycline,tetracycline,ofloxacin and erythromycin were 13.1%,21.3%,32.8%,39.3%,0%, 0%,8.9%,17.8%,17.8% and 48.9%,respectively;and the resistance rates of 41 strains of Mh to minocy-ine,spectinomycin,azithromycin,sparfloxacin,pristinamycin,josamycin,doxycline,tetracycline,ofloxacin and erythromycin were 19.2%,43.3%,42.3%,53.5%,0%,0%,6.7%,17.8%,20.0% and 93.3%,respec- tively.The cblony counts were semiquantitative reference values;treatment was based on clinical symptoms. Conclusions Culture of mycoplasma and drug sensitivity test are conducive to selection of antibiotics and reduc- tion of drug resistant strains in treatment of mycoplasma infection.Culture of mycoplasma from semen,prostat- ic secretion and cervical mucus is recommended.