解脲支原体在1000例已婚女性宫颈分泌物中的检出情况和药敏情况分析

目的:调查分析已婚妇女宫颈分泌物中解脲支原体(Uu)的感染率及药敏情况。方法:收集到本院门诊2013年1月至205年1月进行妇科检查的1000例已婚妇女的宫颈口分泌物,采用荧光定量-聚合酶连反应(QF-PCR)检测分泌物中进行初筛,并采用解脲支原体液体培养基进行接种培养,并进行抗菌药物的药敏试验。结果:1000例患者中共检出353例Uu感染妇女,感染率为35.30%;在不同年龄段检查对象中,26~35岁年龄组妇女的检出率最高46.51%、36~45岁年龄组妇女的检出率为38.28%、46~55岁年龄组妇女的检出率为30.14%。宫颈炎患者的Uu阳性率56.96%显著的高于非宫颈炎患者的25.29%且差异具有统计学意义(P0.05);白带清洁度Ⅰ~Ⅱ度患者的Uu阳性率27.53%显著的低于Ⅲ~Ⅳ度患者的53.51%且差异具有统计学意义(P0.05);宫颈分泌物中Uu检出阳性率与阴道滴虫感染、念珠球菌感染不具有相关性(P0.05)。在10种抗生素耐药实验中,药物敏感率≥90%的有交沙霉素、强力霉素,药物敏感率≥80%的有乙酰螺旋霉素、可乐必妥、左氧氟沙星、阿奇霉素、罗红霉素、加替沙星。结论:已婚妇女的宫颈分泌物中Uu的感染率为35.30%,并且与白带清洁度、宫颈炎有一定的关系,治疗应根据药敏结果进行针对性用药。

Detection and drug sensitivity analysis of ureaplasma urealyticum in cervical secretion in 1000 married women

Objectives:To investigate and analyze the infection rate and drug sensitivity of ureaplasma urealyticum (UU) in cervical secretion of married women.Methods:Cervix secretion of 1000 married women receiving gynecological examination in our hospital from January 2013 to January 2015 was collected.Secretions were screened by fluorescence quantitative polymerase chain reaction (QF-PCR) detection.UU liquid medium was cultured,and drug sensitivity test was done.Results:Among 1000 patients,there were 353 cases of UU infection,an infection rate of 35.30％.In different age group,the detection rate in 26 to 35 year old age group was 46.51％,the detection rate in 36 ～ 45 years old age group was 38.28％ and the detection rate in 46 ～ 55 years old age group was 30.14％.The positive rate of Uu in patients with cervicitis was 56.96％,which was significantly higher than that in non-infected people (25.29％),with statistically significant difference (P ＜ 0.05).The positive rate of Uu in patients with leucorrhea cleanliness Ⅰ-Ⅱ degree was 27.53％,which was obviously lower than that of patients with leucorrhea cleanliness Ⅲ or ⅣV degree (53.51％),with statistically significant difference (P ＜ 0.05).The positive rate of Uu and the detection of Trichomonas vaginalis infection and Candida infection had no correlation (P ＞ 0.05).In the experiment of 10 kinds of antibiotic resistance,the drug sensitive rate of josamycin and doxycycline was greater than 90％.The drug sensitive rate of acetyl spiramycin,ofloxacin,levofloxacin,azithromycin,roxithromycin and gatifloxacin was greater than 80％.Conclusion:UU infection rate in married women is 35.30％.It has a certain relationship with the cleanliness of leucorrhea and cervicitis.The treatment should be carried out according to the drug sensitivity results.