HLA-DRB1等位基因与广东汉族人群甲真菌病的相关性研究

 目的:探讨人类白细胞抗原HLA DRB1等位基因与广东汉族人群甲真菌病的关联性。方法：纳入64例广东汉族甲真菌病患者(包括10例红色毛癣菌甲癣患者)以及64例健康对照者，采用聚合酶链式反应-序列特异性引物（PCR-SSP）技术，对研究对象全血基因DNA进行HLA DRB等位基因分型，比较等位基因频率，分析甲真菌病与HLA基因多态性的关系。结果：甲真菌病患者HLA-DRB1*10基因频率高于健康对照组（ X²=5.10,P<0.05）。红色毛癣菌感染的甲癣患者组HLA DRB1*12基因频率低于健康对照组（  X²=4.70,P<0.05）。结论：HLA-DRB1*10等位基因可能是广东汉族人群甲真菌病遗传易感基因，而HLA-DRB1*12等位基因可能是红色毛癣菌感染的甲真菌病患者的保护性基因，HLA基因多态性与甲真菌病的发生可能存在遗传免疫关联性。

Relationship between HLA DRB1 alleles and onychomycosis in Chinese Han population in Guangdong province

Objective：To investigate the relationship between polymorphism of human leukocyte antigen (HLA) DRB1 alleles and onychomycosis in Chinese Han population in Guangdong province. Methods: Polymerase chain reaction sequence specific primers (PCR SSP) technique was adopted to detect the allele frequencies of HLA DRB1 in 64 cases of onychomycosis, including 10 cases of Trichophyton rubrum infection, and 64 healthy controls. The differences in allele frequencies were compared between patients and controls. Results: The allele frequencies of HLA DRB1*10 in Han patients with onychomycosis were higher than that in the controls (  X²=5.10,P<0.05). In contrast, the allele frequencies of HLA DRB1*12 in Han patients with trichophyton rubrum infection were lower than that in the controls (X²=4.70,P<0.05). Conclusions: HLA DRB1*10 alleles may be the susceptibility gene of onychomycosis in Chinese Han population in Guangdong province. HLA-DRB1*12 alleles could prevent the development of onychomycosis caused by trichophyton rubrum. HLA alleles could be immunogenetically associated with onychomycosis.