131例神经系统疾病患者血清中抗BP180和抗BP230抗体的检测

目的：检测神经系统疾病患者血清中抗BP180、抗BP230和抗基底膜带抗体的阳性率。方法：收集神经系统疾病患者和正常对照血清，采用酶联免疫吸附试验（ELISA）和间接免疫荧光（IIF）检测两组血清中抗BPl80NC16A抗体和BP230抗体水平。结果：共收集到131例神经系统疾病患者血清(脑卒中109例，脑肿瘤17例，其他神经系统疾病19例)和131例正常对照血清。病例组中抗BP180NC16A1阳性率为1.45%，低于对照组的3.05%，差异具有统计学差异（P = 0.009），病例组中抗BP230抗体阳性率5.34%与对照组（2.29%）比较，差异无统计学意义。IIF检测抗基底膜带抗体结果均为阴性。结论：抗BP180NC16A抗体在神经系统疾病患者中有较高的阳性率，可能与神经系统疾病患者合并大疱性类天疱疮相关。

Anti-BP180 and 230 antibodies in 131 patients with neurological diseases

Objective: To detect the level of anti-BP180 and 230 antibodies in the patients with neurological diseases (ND) , but without BP. Methods: The serum and clinical data of the patients with ND and healthy controls were collected. The levels of anti-BP 180, 230 antibodies and anti-basement membrane antibodies were detected by enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence (IIF) respectively. Results: One hundred and thirty-one patients with ND (109 with cerebral apoplexy, 17 with brain tumors and 19 with other neurological disease) and 131 healthy controls were enrolled. Fifteen patients (11.45%) and 4 controls (3.05%) were positive for anti-BP180, NC16A antibodies, with a significant difference of P=0.009. Seven patients (5.34%) and 3 controls (2.29%) were positive for anti-BP 230 antibodies, with no significant difference (P=0.20). The anti-basement membrane antibodies were negative in the patients and healthy controls. Conclusion: The positivity rate of anti-BP180 antibodies was higher in the patients with neurological diseases, which may be associated with the pathogenesis of ND complicated with BP.