TL1A及其受体DcR3在寻常型银屑病皮损中的表达

目的:检测TL1A及其受体DcR3在寻常型银屑病患者皮损组织中的表达。方法:采用免疫组织化学法和实时荧光定量RT-PCR法对30例寻常型银屑病患者皮损中TL1A和DcR3水平进行检测。结果:银屑病组中TL1A和DcR3蛋白阳性表达率分别为93.3%和90%,正常对照组中TL1A和DcR3蛋白阳性表达率分别为20%和60%;银屑病组TL1A mRNA和DcR3 mRNA表达CT值分别为4.909±1.607和3.430±1.449,正常对照组TL1A mRNA和DcR3 mRNA表达CT值分别为1.250±0.650和1.011±0.150,银屑病组中TL1A和DcR3蛋白及mRNA水平明显高于正常对照,差异均有统计学意义。银屑病皮损中TL1A与DcR3的表达以及TL1A mRNA与DcR3 mRNA蛋白的表达均呈正相关性(r=0.735,P0.01和r=0.2436,P0.05)。结论:TL1A和DcR3可能参与银屑病炎症的发生机制。

Expression of TL1A and its receptor DcR3 in the skin lesions of psoriasis vulgaris

Objective:To investigate the expression of TNF-like ligand 1 aberrance ( TL1A) and its re-ceptor decoy receptor3 (DcR3) in the skin lesions of the patients with psoriasis vulgaris (PV). Methods:The expression of TL1A and DcR3 in the skin lesions of 30 patients with PV was tested with SP immunohisto-chemical and real-time fluorescence quantitative RT-PCR method. Results:The positivity rates of TL1A and DcR3 proteins were 93.3% and 90% in PV, and were 20% and 60% in the normal samples. The expression levels of TL1A mRNA and DcR3 mRNA were (4.909±1.607) and (3.430±1.449) in PV, and (1.250± 0.650) and (1.011±0.150) in normal skin. The level of TL1A and DcR3 protein and mRNA were significant-ly higher in PV than that in normal tissue, with a significant difference. There was a positively correlation be-tween the protein level of TL1A and DcR3 in PS ( P<0.01); TL1A mRNA and DCR3 mRNA expression in psoriatic lesion was a positive correlation ( P<0.05);TL1A mRNA expression in PS and PASI shows no corre-lation ( P>0.05 ); DcR3 mRNA expression in PS was positively correlation with PASI ( P<0. 05 ) . Conclusion:TL1A and DcR3 may participate in the pathogenesis of chronic skin inflammation in psoriasis.