淋球菌体外感染单个核细胞对IL-1β、NLRP3表达的影响

目的：明确不同淋球菌体外感染单个核细胞对IL-1β、NLRP3表达的影响。方法：提取小鼠脾脏单个核细胞，通过利用分光光度计法定量菌液浓度，选取OD450值在0.2、0.6、1.0、1.4的菌液进行稀释，并感染所提取的单个核细胞，共培养6 h后采用实时荧光定量PCR检测各组细胞IL-1β、NLRP3 mRNA表达。结果：实时荧光定量PCR结果显示，IL-1β、NLRP3表达水平均显著高于空白对照组，且出现先升高后下降的趋势，以OD450值=0.6淋球菌悬液感染单核细胞时IL-1β表达最高，而淋球菌悬液OD450值=1.0感染时NLRP3表达最高。结论：淋球菌体外感染单个核细胞可导致其IL-1β、NLRP3的表达明显上调。

Effects of gonococcus on the expression of IL-1β, NLRP3 in monocytes

Objective: To investigate  the expression of IL-1β and NLRP3 in gonococcus infected monocytes. Methods: Mouse spleen mononuclear cells were extracted. The different concentrations of bacterial solutions were identified by spectrophotometer and the concentrations with OD450 of 0.2, 0.6, 1.0 and 1.4 were selected. The extracted mononuclear cells were infected by gonococcus. After co-culture for 6 h, the expression of IL-1β in each group were detected by real-time fluorescent quantitative PCR. Results: The results of real-time quantitative PCR showed that IL-1β, NLRP3 expression levels in the experiment groups were higher than those in the blank control group with the tend of increased in initial stage and then declined. The highest of IL-1β expression level in the mononuclear cells was under the concentration with OD450=0.6, while NLRP3 level when the bacterial solution concentration OD450 was 1.0. Conclusion: Gonococcus can up-regulate the IL-1β and NLRP3 expression in monocytes.