槲皮黄酮通过Sphk2抑制人皮肤鳞状细胞癌A431细胞的增殖

目的：研究槲皮黄酮（Quercetin, Qu）对人皮肤鳞状细胞癌A431细胞增殖活性的影响。方法：采用CCK8法、Western blot实验和流式细胞术检测不同浓度下（10、50、100、200 μg/mL）A431细胞活力、增殖及凋亡相关指标；Western blot检测Qu对A431细胞中Sphk2信号通路的影响。结果：10、50、100、200 μg/mL Qu作用96 h后，细胞OD值分别为（0.654±0.098）、（0.527±0.089）、（0.412±0.076）、（0.309±0.054）；细胞活力分别为（81.3±4.6）%、（56.3±4.9）%、（37.4±4.2）%、（19.4±3.6）%;细胞凋亡率分别为（6.43±1.09）%、（14.77±1.26）%、（21.30±1.36）%、(29.84±1.26)%。Qu呈浓度依赖性的抑制细胞中Sphk2蛋白表达，且Qu+Sphk2 mimics组细胞活力明显高于于Qu，细胞凋亡率明显低于Qu组(Ps<0.05)。结论：Qu可能通过抑制Sphk2信号通路降低人皮肤鳞状细胞癌细胞的增殖活性。

Quercetin inhibits the proliferation of human skin squamous cell carcinoma A431 cells through sphingosine kinase 2

Objective: To study the effects of quercetin (Qu) on the proliferative activity of human skin squamous cell carcinoma cells (A431). Methods: The viability, proliferation and apoptosis of A431 cells treated by QU of different concentration (10, 50, 100 and 200 μg/mL) were respectively detected by CCK-8 assay, Western blot and flow cytometry assay. The impacts of Qu on activation of sphingosine kinase 2 (Sphk2) in A431 cells were detected by Western blot. Results: After treated for 96h by QU of concentration 10, 50, 100 and 200 μg/mL, the OD readings were (0.654±0.098),（0.527±0.089）,（0.412±0.076）and（0.309±0.054）, respectively; the cell activities were (81.3±4.6)%, (56.3±4.9)%, (37.4±4.2)%, (19.4±3.6)% , respectively and the apoptosis rate of A431 cells were (6.43±1.09)%, (14.77±1.26)%,（21.30±1.36）% and (29.84±1.26)% , respectively. Qu dose-dependently inhibited the expression levels of Sphk2 in A431 cells, and the cell activity of A431 cells in Qu+Sphk2 mimics was higher and the apoptosis rate was lower than that in Qu group (Ps<0.05). Conclusion: Qu could inhibit the proliferative activity of human skin squamous cell carcinoma cells through decreasing the activation of Sphk2 pathway.