抑制树突细胞MMP-13基因对咪喹莫特诱导的小鼠银屑病样炎症模型的影响

目的：明确抑制树突细胞MMP-13基因对咪喹莫特诱导的小鼠银屑病样炎症模型的影响。方法：构建树突细胞MMP-13基因特异性敲除的小鼠模型（MMP-13^LOX/LOX），应用咪喹莫特诱导小鼠银屑病样皮损的发生。将小鼠分为野生型对照组、MMP-13^LOX/LOX对照组、咪喹莫特诱导的野生型实验组和咪喹莫特诱导的MMP-13^LOX/LOX实验组，分析不同组别小鼠皮损临床表现并记录PASI评分，检测各组小鼠皮损中IL-1β、IL-6、IL-23和IL-17A表达。结果：与咪喹莫特诱导的野生型实验组小鼠比较，咪喹莫特诱导的MMP-13^LOX/LOX实验组银屑病皮损表现相比更加温和，仅有轻度的棘层肥厚和真皮细胞浸润，皮损中IL-1β、IL-6表达下调，而IL-23和IL-17A表达无明显改变。结论：抑制树突细胞MMP-13基因可降低炎症因子IL-6、IL-1β的表达及减弱小鼠皮肤银屑病炎症反应。

The effect of inhibition of MMP-13 gene in dendritic cell on a mouse model of imiquimod-induced psoriasis

Objective:To determine the effect of inhibition of MMP-13 gene in dendritic cell on a mouse model of imiquimod-induced psoriasis.Methods:A mouse model of MMP-13 gene specific knockout in dendritic cells was constructed,and then,the psoriasis lesion was induced by imiquimod.Mice were divided into wild-type control group,MMP-13 LOX/LOX control group,wild-type imiquimod group,MMP-13 LOX/LOX imiquimod group.The clinical manifestations of skin lesions in each group and the psoriasis severity index(PASI)were assessed.The expressions of IL-1,IL-6,IL-23 and IL-17A in mice lesions were detected.Results:Compared with the wild-type imiquimod group,the lesions manifestation of the mice in MMP-13 LOX/LOX imiquimod group were milder,with only mild acanthosis and dermal cell infiltration.The expression level of IL-1 and IL-6 was lower and there was no significant difference in the expression level of IL-23 and IL-17A.Conclusion:Inhibition of MMP-13 gene of dendritic cells can decrease the level of IL-6 and IL-1β and inhibit the inflammation of psoriasis mouse model.