| HPV18 E7基因的原核表达及其在宫颈癌患者血清抗体中的检测 |
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| 引用本文： | 吴永琴,陈军,朱珊丽,孟锐锋,陆丽君,张丽芳.HPV18 E7基因的原核表达及其在宫颈癌患者血清抗体中的检测[J].中国皮肤性病学杂志,2010,24(1). |
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| 作者姓名： | 吴永琴 陈军 朱珊丽 孟锐锋 陆丽君 张丽芳 |
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| 作者单位： | 浙江省温州医学院微生物学与免疫教研室,分子病毒与免疫研究所,浙江,温州,325035 |
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| 摘 要： | 目的研究人类乳头瘤病毒18型E7全长基因(HPV18 E7)在原核表达系统中的表达,及其作为特异性抗原在宫颈癌血清抗体检测中的意义,为HPV感染与肿瘤相关的血清学诊断研究奠定基础。方法PCR法扩增HPV18 E7全长基因,构建重组质粒pET32a(+)/HPV18 E7,转化表达HPV18 E7融合蛋白后经纯化、SDS-PAGE和Western blot分析鉴定。再以该融合蛋白作为诊断抗原,间接ELISA法检测宫颈癌、尖锐湿疣及健康对照三组血清特异性IgG抗体水平。结果HPV18 E7融合蛋白表达量约占菌体总蛋白的35.00%;宫颈癌、尖锐湿疣及健康对照组的血清特异性抗体均值分别为0.604±0.328,0.287±0.202和0.342±0.225,三组间差异有显著性意义(F=32.771,P<0.01);宫颈癌组与尖锐湿疣组及健康对照组的HPV18E7均值比较,差异均有显著性意义(P均<0.01),而尖锐湿疣组与健康对照组抗体均值比较,差异无统计学意义(P>0.05)。结论HPV18 E7融合蛋白具较强的抗原性,对宫颈癌诊断试剂的研究具有应用价值。
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| 关 键 词： | HPV18 E7融合蛋白 宫颈癌 ELISA 血清特异性抗体 |
The Expression of the Full-length Gene of Human Papillomavirus Type 18 E7 in Prokaryotic Cells and Its Serum Antibody in Patients with Cervical Cancer |
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| WU Yong-qin,CHEN Jun,ZHU Shan-li,MENG Rui-feng,LU Li-jun,ZHANG Li-fang.The Expression of the Full-length Gene of Human Papillomavirus Type 18 E7 in Prokaryotic Cells and Its Serum Antibody in Patients with Cervical Cancer[J].The Chinese Journal of Dermatovenereology,2010,24(1). |
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| Authors: | WU Yong-qin CHEN Jun ZHU Shan-li MENG Rui-feng LU Li-jun ZHANG Li-fang |
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| Institution: | WU Yong-qin,CHEN Jun,ZHU Shan-li,MENG Rui-feng,LU Li-jun,ZHANG Li-fang(Department of Microbiology , Immunology,School of Basic Medical Science,Wenzhou Medical College,The institute of Molecular virology , immunology,Wenzhou 325035,China) |
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| Abstract: | Objective To study the expression of full-length oncoproteins of human papillomavirus type 18 E7 in prokaryotic expression system,and to screen the serum antibody in the patients with cervical cancer by antigen of the recombinant HPV 18 E7 protein,try to establish the method for the serum diagnosis of HPV infection and the HPV associated disease.Methods HPV18 E7 full-length oncoproteins was amplified by PCR and then cloned into the vector pET32a(+)to construct the recombinant plasmid pET32a(+)/HPV 18E7,then... |
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| Keywords: | ELISA |
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