应用A7琼脂法检测泌尿生殖道支原体

目的：评价A7琼脂法在检测泌尿生殖道标本中解脲脲原体(Ureaplasma urealyticum,Un)和人型支原体(Mycoplsma hominis，Mh)中的意义，为临床诊断Uu和Mh的感染提供实验依据。方法：取接种了泌尿生殖道标本的运送培养基直接接种于A7琼脂，在5％CO2和95％N2的气体环境中培养24h～48h后，低倍镜下观察特征性菌落，计数其菌落形成单位(Colony Forming Units,CFU)。结果：286例泌尿生殖道标本中分离到Uu 102例，Mh29例，混合感染27例，Uu计数≥10^4CFU75例，Mh计数≥10^4CFU24例。结论：A7琼脂法简便可行，结果直观可信，适用于临床泌尿生殖道中Uu、Mh的检测。

Detection of Urinary Mycoplasma by Using A7 Agar Medium Method

Objective:To evaluate the A7 agar medimn method to be applied to detect ureaplasma urealyticum(Uu) and mycoplasma hominis(Mh) in urinary tract and provide laboratory test for clinical diagnosis. Methods:Shipping medium with clinical specimens in it was inoculated in A7 agar plate. Incubated under 5% CO 2/95% N 2 for 24 to 48 hours,specific colonies were observed by microscope and colony forming units(CFU) were counted.Results: Of all 286 samples studied, 102 U. urealytlcum,29 M. hominis and 27 co-infection have been isolated in A7 agar. U. urealyticum CFU of 75 cases were more than or equal to 10 4 and M. hominis CFU of 24 cases were more than or equal to 10 4. Conclusion:A7 agar medium method is asimple and feasible method for clinical detection of U. urealyticum and M. hominis in urinary tract samples.