固相配体文库技术分析胰腺癌差异血清蛋白表达谱的研究

 目的：通过胰腺癌患者血清差异蛋白表达谱鉴定胰腺癌的血清学生物标志物。 方法：应用固相配体文库技术处理胰腺癌及非癌对照的血清，然后采用二维电泳技术分析差异点、串联质谱鉴定与胰腺癌变相关的血清差异蛋白质分子；应用ELISA检测凝集素在胰腺癌血清中的表达情况。 结果: 7种蛋白质分子在胰腺癌患者血清中的表达量升高，包括凝集素（clusterin）, 玻连蛋白（vitronectin）,补体C4-A( complement C4-A), 维生素蛋白D结合蛋白（vitamin D-binding protein）,抗凝血酶III（ anitthrombin-III）, 补体C6（complement component C6）, 凝血酶原（prothrombin）；补体C7（complement component C7）血清表达量下降。ELISA检测结果表明胰腺癌患者血清凝集素水平显著高于非癌对照（P<0.05）。 结论: 凝集素可能与胰腺癌的发生发展过程密切相关，血清凝集素检测可能是重要的胰腺癌诊断生物标志物。

Serum proteome profiling of pancreatic adenocarcinoma

Objective To identify biomarkers involved in pancreatic adenocarcinoma by serum proteome fingerprinting.Methods ProteoMiner beads were used to treat neat serum sample of pancreatic cancer and control tissues followed by 2-D gel electrophoresis and ESI-MS/MS to identify the signature proteins involved in pancreatic carcinogenesis.ELISA was used to determine serum level of clusterin.Results Seven proteins including clusterin,vitronectin,complement C4-A,vitamin D-binding protein,anitthrombin-Ⅲ,complement component C and prothrombin were up-regulated and complement component C7 was down-regulaed in serum of pancreatic cancer.ELISA results showed that serum level of clusterin enhanced in pancreatic adenocarcinoma significantly(P<0.05).ConclusionsClusterin may correlate closely with development and progression of pancreatic adenocarcinoma and serum level of clusterin may serve as a key biomarker for diagnosis of pancreatic adenocarcinoma.