骨髓间充质干细胞移植促进大鼠子宫创伤修复

 摘要 目的 探讨移植骨髓间充质干细胞移植（BM-MSCs）后在子宫切口部位的生长分化，及其在大鼠子宫创伤后再生修复中的作用。方法 分离和培养大鼠BM-MSCs，传代扩增至第4代利用腺病毒-绿色荧光蛋白感染干细胞并继续培养。取雌性SD大鼠40只，分为对照组及实验组，建立子宫损伤动物模型， 实验组移植BM-MSCs，对照组注射生理盐水，2周后观察干细胞在移植部位的生长、分化,并通过检测局部CTGF、CD34的表达及Masson染色的情况比较瘢痕愈合情况。结果 移植后的干细胞在移植部位能很好的生长，并可向平滑肌细胞方向分化。在一定程度上可以降低局部的胶原纤维沉积，减轻瘢痕愈合。试验组CTGF的表达及Masson染色分别为0.0706±0.0088及87.0±10.3，显著低于对照组的0.0842±0.0146及136.0±15.2，结论 干细胞移植可以在一定程度上减轻子宫的瘢痕愈合，促进子宫的功能性修复。

Bone marrow mesenchymal stem cells transplantation promotes regeneration repair of uterine incision in rat

Objective To investigate the growth and differentiation of adenovirus-green fluorescent protein labelled bone marrow mesenchymal stem cells(BM-MSCs) in uterus incision,and to discuss its potential regeneration repair effect on uterine incision in rat.Methods BM-MSCs were collected by density gradient centrifugation and cultured for fourth generations,then transfected and labelled with adenovirus-green fluorescent protein.Fourty female SD rats were divided equally into control and experimental group,followed with the establishment of uterine wound model.After two weeks,adenovirus-green labelled BM-MSCs were transplanted directly into uterus at previous incision,with the control group only received normal saline injection.Then,one month later,CTGF,CD34 and Masson were used to detect the outcome of the transplanted BM-MSCs.Results The transplanted BM-MSCs can growth well and showed some extent of differentiation toward smooth muscle cells.Significant difference existed for CTGF and Masson between experimental and control samples(both P<0.01).Conclusions BM-MSCs transplantation is a promising technique and can be used to promote regeneration repair of uterine incision.