| Cajal间质细胞数量在反复膀胱炎大鼠膀胱中增加 |
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| 引用本文： | 高峰,张士更,张楠,文甲明,虞旗旗,黄亚胜.Cajal间质细胞数量在反复膀胱炎大鼠膀胱中增加[J].基础医学与临床,2020,40(9):1237-1241. |
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| 作者姓名： | 高峰 张士更 张楠 文甲明 虞旗旗 黄亚胜 |
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| 作者单位： | 杭州市中医院 泌尿外科,浙江 杭州310007;浙江大学 医学院附属第二医院 泌尿外科,浙江 杭州310009 |
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| 摘 要： | 目的研究Cajal间质细胞(ICCs)在大鼠反复膀胱炎相关膀胱过度活动症(OAB)膀胱中的数量变化。方法将大鼠分为对照组和实验组大肠埃希菌(E.coli)1×10^8~1.2×10^8 CFU/mL,100μL膀胱灌注],通过尿检和尿流动力学测定及HE染色膀胱组织明确OAB建模成功;用免疫荧光法观察膀胱组织c-kit阳性的ICCs,Western blot检测干细胞因子(SCF)及c-kit蛋白表达水平。结果实验组大鼠较对照组ICCs网格状分布紊乱,数量明显增加(P<0.05),干细胞因子(SCF)及c-kit蛋白表达明显升高(P<0.05)。结论ICCs在反复膀胱炎膀胱中明显升高。
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| 关 键 词： | 膀胱过度活动症 卡哈尔(Cajal)间质细胞 干细胞因子 c-kit受体酪氨酸激酶 |
Number increase of Cajal interstitial cells in bladder of rats with recurrent cystitis |
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| GAO Feng,ZHANG Shi-geng,ZHANG Nan,WEN Jia-ming,YU Qi-qi,HUANG Ya-sheng.Number increase of Cajal interstitial cells in bladder of rats with recurrent cystitis[J].Basic Medical Sciences and Clinics,2020,40(9):1237-1241. |
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| Authors: | GAO Feng ZHANG Shi-geng ZHANG Nan WEN Jia-ming YU Qi-qi HUANG Ya-sheng |
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| Institution: | (Department of Urology,Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou 310007;Department of Urology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China) |
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| Abstract: | Objective To explore the changes of interstitial cells of Cajal(ICCs)in the over active bladder(OAB)rats caused by recurrent cystitis.Methods Rats were divided into control group and experimental group(E.coli 1×10^8~1.2×10^8 CFU/mL,100μL bladder perfusion),The OAB model was confirmed by urine test,urine flow dynamics and HE staining of bladder tissue.The immunofluorescence staining method was performed to detect c-kit positive ICCs in bladder tissue.Western blot was used to determine the expression of SCF/c-kit protein.ResultsCompared with the control group,the quantity of ICCs in the experimental group increased significantly(P<0.05),and the protein expression of SCF and c-kit increased significantly(P<0.05).Conclusions ICCs increase significantly in the bladder with recurrent cystitis. |
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| Keywords: | over active bladder interstitial cells of Cajal stem cell factor(SCF) c-kit receptor tyrosine kinase |
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